@inbook{c0de5693cbd845819dcb30ab06bbe75f,
title = "AAV-Mediated Somatic Gene Editing for Cardiac and Skeletal Muscle in a Large Animal Model",
abstract = "Here we describe a protocol to produce a recombinant adeno-associated viral vector (rAAV)-based system to deliver the CRISPR-Cas9 complex into porcine skeletal muscle and myocardial cells. We initially describe the genomic composition of the rAAV-CRISPR vectors used in our lab. Furthermore, we give a step-by-step instruction into the production of recombinant viral vectors with high yields and purity. Lastly we describe the minimally invasive injection regimes to target the myocardium in a pig.",
keywords = "CRISPR, Cas9, Duchenne muscular dystrophy, Gene editing, Gene vector, rAAV",
author = "Tilman Ziegler and Tarik Bozoglu and Christian Kupatt",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2707-5\_6",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "63--74",
booktitle = "Methods in Molecular Biology",
}