A stably transfected c-myc cat hybrid gene is not regulated by serum in NIH3T3 cells

Michaela Götschl, Dirk Eick

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A plasmid containing the CAT (chloramphenicolacetyltransferase) gene fused to the 5′ adjacent sequences and first exon of the human c-myc gene was stably transfected into NIH3T3 cells. Single cell clones were grown and CAT activity was measured after serum starvation and stimulation. CAT activity of the hybrid construct remained unchanged in serum-deprived and serum-stimulated cells. In contrast the steady-state RNA level of the endogenous mouse c-myc gene was strongly elevated upon serum stimulation. The bona fide usage of the human c-myc promoter P1/P2 in mouse cells carrying the hybrid gene was revealed by S1 analysis.

Original languageEnglish
Pages (from-to)379-388
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume157
Issue number1
DOIs
StatePublished - 30 Nov 1988
Externally publishedYes

Fingerprint

Dive into the research topics of 'A stably transfected c-myc cat hybrid gene is not regulated by serum in NIH3T3 cells'. Together they form a unique fingerprint.

Cite this