A simple way for sequential assignment in isotopically enriched proteins using a H(N)CACO correlation

Stephan Seip; Jochen Balbach; Horst Kessler

doi:10.1007/BF00178265

A simple way for sequential assignment in isotopically enriched proteins using a H(N)CACO correlation

Stephan Seip, Jochen Balbach, Horst Kessler

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Technical University of Munich

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Abstract

A simplified scheme for sequential assignment in isotopically enriched proteins is presented. It is based on the standard triple resonance experiments HNCO, HN(CO)CA, HNCA and a modified H(N)CACO correlation, in which both of the H^N connectivities to the CA/C′ pair of residue i and i-1 are observed. The H(N)CACO was tested on uniformly ¹³C/¹⁵N enriched P13 domain of mannose permease (31 kDa).

Original language	English
Pages (from-to)	233-237
Number of pages	5
Journal	Journal of Biomolecular NMR
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/BF00178265
State	Published - Mar 1993

Keywords

Isotope labeling
Mannose permease
Proteins
Sequential assignment
Triple resonance

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10.1007/BF00178265

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abstract = "A simplified scheme for sequential assignment in isotopically enriched proteins is presented. It is based on the standard triple resonance experiments HNCO, HN(CO)CA, HNCA and a modified H(N)CACO correlation, in which both of the HN connectivities to the CA/C′ pair of residue i and i-1 are observed. The H(N)CACO was tested on uniformly 13C/15N enriched P13 domain of mannose permease (31 kDa).",

keywords = "Isotope labeling, Mannose permease, Proteins, Sequential assignment, Triple resonance",

author = "Stephan Seip and Jochen Balbach and Horst Kessler",

year = "1993",

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AU - Seip, Stephan

AU - Balbach, Jochen

AU - Kessler, Horst

PY - 1993/3

Y1 - 1993/3

N2 - A simplified scheme for sequential assignment in isotopically enriched proteins is presented. It is based on the standard triple resonance experiments HNCO, HN(CO)CA, HNCA and a modified H(N)CACO correlation, in which both of the HN connectivities to the CA/C′ pair of residue i and i-1 are observed. The H(N)CACO was tested on uniformly 13C/15N enriched P13 domain of mannose permease (31 kDa).

AB - A simplified scheme for sequential assignment in isotopically enriched proteins is presented. It is based on the standard triple resonance experiments HNCO, HN(CO)CA, HNCA and a modified H(N)CACO correlation, in which both of the HN connectivities to the CA/C′ pair of residue i and i-1 are observed. The H(N)CACO was tested on uniformly 13C/15N enriched P13 domain of mannose permease (31 kDa).

KW - Isotope labeling

KW - Mannose permease

KW - Proteins

KW - Sequential assignment

KW - Triple resonance

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U2 - 10.1007/BF00178265

DO - 10.1007/BF00178265

M3 - Article

AN - SCOPUS:34250083733

SN - 0925-2738

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EP - 237

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 2

ER -

A simple way for sequential assignment in isotopically enriched proteins using a H(N)CACO correlation

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Keywords

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