Abstract
A sensitive enzyme immunoassay for the detection of atrazine has been developed on the basis of sheep antibodies. Sheep were immunized with an immunogen prepared by coupling 2-aminohexanecarboxylic acid-4-isopropylamino-6-ethylamino-1,3,5-triazine to keyhole limpet hemocyanin. They produced antibodies with high affinity for atrazine. An enzyme immunoassay (EIA) was optimized with a peroxidase-atrazine tracer on microtitre plates. The assay with a detection limit of 0.03 ppb permits direct measurement of water samples without concentration or clean-up steps. Cross-reactivities above 10 % were only detected for propazine, which is not used in most European countries. Validation showed good correspondence of EIA and GC/MS with a correlation coefficient of r = 0.982.
| Original language | English |
|---|---|
| Pages (from-to) | 1025-1037 |
| Number of pages | 13 |
| Journal | Analytical Letters |
| Volume | 25 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1 Jun 1992 |
Keywords
- Atrazine
- enzyme immunoassay
- KLH
- sheep antibodies