A secreted enzyme reporter system for MRI

Gil G. Westmeyer; Yves Durocher; Alan Jasanoff

doi:10.1002/anie.200906712

A secreted enzyme reporter system for MRI

Gil G. Westmeyer
, Yves Durocher
, Alan Jasanoff

Massachusetts Institute of Technology
National Research Council of Canada

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

(Figure Presented) Let's see what comes out: An extracellular enzymatic gene-reporter system for magnetic resonance imaging (MRI) yields strong, reversible contrast changes in response to the expression of secreted alkaline phosphatase (SEAP; see picture). Products of SEAP activity were specifically detected using an iron-oxide-based sensor. The contrast agent is not used up by the enzyme, cell delivery is not required, and multimodal detection is possible.

Original language	English
Pages (from-to)	3909-3911
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	23
DOIs	https://doi.org/10.1002/anie.200906712
State	Published - 25 May 2010
Externally published	Yes

Keywords

Alkaline phosphatase
Aptamers
Enzymatic reporters
Gene reporters
Magnetic resonance imaging

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10.1002/anie.200906712

Cite this

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abstract = "(Figure Presented) Let's see what comes out: An extracellular enzymatic gene-reporter system for magnetic resonance imaging (MRI) yields strong, reversible contrast changes in response to the expression of secreted alkaline phosphatase (SEAP; see picture). Products of SEAP activity were specifically detected using an iron-oxide-based sensor. The contrast agent is not used up by the enzyme, cell delivery is not required, and multimodal detection is possible.",

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AU - Durocher, Yves

AU - Jasanoff, Alan

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N2 - (Figure Presented) Let's see what comes out: An extracellular enzymatic gene-reporter system for magnetic resonance imaging (MRI) yields strong, reversible contrast changes in response to the expression of secreted alkaline phosphatase (SEAP; see picture). Products of SEAP activity were specifically detected using an iron-oxide-based sensor. The contrast agent is not used up by the enzyme, cell delivery is not required, and multimodal detection is possible.

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KW - Alkaline phosphatase

KW - Aptamers

KW - Enzymatic reporters

KW - Gene reporters

KW - Magnetic resonance imaging

UR - https://www.scopus.com/pages/publications/77952977376

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M3 - Article

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

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A secreted enzyme reporter system for MRI

Abstract

Keywords

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