TY - JOUR
T1 - A review of standardized metabolic phenotyping of animal models
AU - Rozman, Jan
AU - Klingenspor, Martin
AU - Hrabě de Angelis, Martin
N1 - Publisher Copyright:
© 2014, Springer Science+Business Media New York.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Metabolic phenotyping of genetically modified animals aims to detect new candidate genes and related metabolic pathways that result in dysfunctional energy balance regulation and predispose for diseases such as obesity or type 2 diabetes mellitus. In this review, we provide a comprehensive overview on the technologies available to monitor energy flux (food uptake, bomb calorimetry of feces and food, and indirect calorimetry) and body composition (qNMR, DXA, and MRI) in animal models for human diseases with a special focus on phenotyping methods established in genetically engineered mice. We use an energy flux model to illustrate the principles of energy allocation, describe methodological aspects how to monitor energy balance, and introduce strategies for data analysis and presentation.
AB - Metabolic phenotyping of genetically modified animals aims to detect new candidate genes and related metabolic pathways that result in dysfunctional energy balance regulation and predispose for diseases such as obesity or type 2 diabetes mellitus. In this review, we provide a comprehensive overview on the technologies available to monitor energy flux (food uptake, bomb calorimetry of feces and food, and indirect calorimetry) and body composition (qNMR, DXA, and MRI) in animal models for human diseases with a special focus on phenotyping methods established in genetically engineered mice. We use an energy flux model to illustrate the principles of energy allocation, describe methodological aspects how to monitor energy balance, and introduce strategies for data analysis and presentation.
UR - http://www.scopus.com/inward/record.url?scp=84929517148&partnerID=8YFLogxK
U2 - 10.1007/s00335-014-9532-0
DO - 10.1007/s00335-014-9532-0
M3 - Review article
C2 - 25199945
AN - SCOPUS:84929517148
SN - 0938-8990
VL - 25
SP - 497
EP - 507
JO - Mammalian Genome
JF - Mammalian Genome
IS - 9-10
ER -