A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein

Akira Tsugita; Toyoaki Uchida; H. Werner Mewes; Tatsuaki Ataka

doi:10.1093/oxfordjournals.jbchem.a122209

A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein

Akira Tsugita
, Toyoaki Uchida
, H. Werner Mewes
, Tatsuaki Ataka

Tokyo University of Science
Max Planck Institute of Biochemistry
Seiko Instruments Inc

Research output: Contribution to journal › Article › peer-review

112 Scopus citations

Abstract

A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HGl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158°C for reaction times of 22.5 and 45 min with 7 M HC1 and 10% TFA containing 0.1 % phenol.

Original language	English
Pages (from-to)	1593-1597
Number of pages	5
Journal	Journal of Biochemistry
Volume	102
Issue number	6
DOIs	https://doi.org/10.1093/oxfordjournals.jbchem.a122209
State	Published - Dec 1987
Externally published	Yes

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title = "A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein",

abstract = "A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HGl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158°C for reaction times of 22.5 and 45 min with 7 M HC1 and 10\% TFA containing 0.1 \% phenol.",

author = "Akira Tsugita and Toyoaki Uchida and Mewes, \{H. Werner\} and Tatsuaki Ataka",

year = "1987",

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doi = "10.1093/oxfordjournals.jbchem.a122209",

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AU - Tsugita, Akira

AU - Uchida, Toyoaki

AU - Mewes, H. Werner

AU - Ataka, Tatsuaki

PY - 1987/12

Y1 - 1987/12

N2 - A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HGl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158°C for reaction times of 22.5 and 45 min with 7 M HC1 and 10% TFA containing 0.1 % phenol.

AB - A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HGl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158°C for reaction times of 22.5 and 45 min with 7 M HC1 and 10% TFA containing 0.1 % phenol.

UR - https://www.scopus.com/pages/publications/0023498598

U2 - 10.1093/oxfordjournals.jbchem.a122209

DO - 10.1093/oxfordjournals.jbchem.a122209

M3 - Article

C2 - 2834350

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JF - Journal of Biochemistry

IS - 6

ER -

A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein

Abstract

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