A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation

Pavel Kielkowski, Isabel Y. Buchsbaum, Tobias Becker, Kathrin Bach, Silvia Cappello, Stephan A. Sieber

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide–alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.

Original languageEnglish
Pages (from-to)1285-1287
Number of pages3
JournalChemBioChem
Volume21
Issue number9
DOIs
StatePublished - 4 May 2020

Keywords

  • AMPylation
  • click chemistry
  • fluorescent probes
  • post-translational modifications
  • proteomics

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