A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation

Pavel Kielkowski; Isabel Y. Buchsbaum; Tobias Becker; Kathrin Bach; Silvia Cappello; Stephan A. Sieber

doi:10.1002/cbic.201900716

A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation

Pavel Kielkowski, Isabel Y. Buchsbaum, Tobias Becker, Kathrin Bach, Silvia Cappello, Stephan A. Sieber

Chair of Organic Chemistry II

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide–alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.

Original language	English
Pages (from-to)	1285-1287
Number of pages	3
Journal	ChemBioChem
Volume	21
Issue number	9
DOIs	https://doi.org/10.1002/cbic.201900716
State	Published - 4 May 2020

Keywords

AMPylation
click chemistry
fluorescent probes
post-translational modifications
proteomics

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10.1002/cbic.201900716

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abstract = "Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide–alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.",

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AU - Kielkowski, Pavel

AU - Buchsbaum, Isabel Y.

AU - Becker, Tobias

AU - Bach, Kathrin

AU - Cappello, Silvia

AU - Sieber, Stephan A.

PY - 2020/5/4

Y1 - 2020/5/4

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AB - Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide–alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.

KW - AMPylation

KW - click chemistry

KW - fluorescent probes

KW - post-translational modifications

KW - proteomics

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ER -

A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation

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Keywords

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