A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis

Julia Legen, Benjamin Lenzen, Nitin Kachariya, Stephanie Feltgen, Yang Gao, Simon Mergenthal, Willi Weber, Enrico Klotzsch, Reimo Zoschke, Michael Sattler, Christian Schmitz-Linneweber

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Arabidopsis (Arabidopsis thaliana) plants can produce photosynthetic tissue with active chloroplasts at temperatures as low as 4°C, and this process depends on the presence of the nuclear-encoded, chloroplast-localized RNA-binding protein CP29A. In this study, we demonstrate that CP29A undergoes phase separation in vitro and in vivo in a temperature-dependent manner, which is mediated by a prion-like domain (PLD) located between the two RNA recognition motif domains of CP29A. The resulting droplets display liquid-like properties and are found near chloroplast nucleoids. The PLD is required to support chloroplast RNA splicing and translation in cold-treated tissue. Together, our findings suggest that plant chloroplast gene expression is compartmentalized by inducible condensation of CP29A at low temperatures, a mechanism that could play a crucial role in plant cold resistance.

Original languageEnglish
Pages (from-to)2851-2872
Number of pages22
JournalPlant Cell
Volume36
Issue number8
DOIs
StatePublished - Aug 2024

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