TY - JOUR
T1 - A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis
AU - Legen, Julia
AU - Lenzen, Benjamin
AU - Kachariya, Nitin
AU - Feltgen, Stephanie
AU - Gao, Yang
AU - Mergenthal, Simon
AU - Weber, Willi
AU - Klotzsch, Enrico
AU - Zoschke, Reimo
AU - Sattler, Michael
AU - Schmitz-Linneweber, Christian
N1 - Publisher Copyright:
© 2024 The Author(s). Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved.
PY - 2024/8
Y1 - 2024/8
N2 - Arabidopsis (Arabidopsis thaliana) plants can produce photosynthetic tissue with active chloroplasts at temperatures as low as 4°C, and this process depends on the presence of the nuclear-encoded, chloroplast-localized RNA-binding protein CP29A. In this study, we demonstrate that CP29A undergoes phase separation in vitro and in vivo in a temperature-dependent manner, which is mediated by a prion-like domain (PLD) located between the two RNA recognition motif domains of CP29A. The resulting droplets display liquid-like properties and are found near chloroplast nucleoids. The PLD is required to support chloroplast RNA splicing and translation in cold-treated tissue. Together, our findings suggest that plant chloroplast gene expression is compartmentalized by inducible condensation of CP29A at low temperatures, a mechanism that could play a crucial role in plant cold resistance.
AB - Arabidopsis (Arabidopsis thaliana) plants can produce photosynthetic tissue with active chloroplasts at temperatures as low as 4°C, and this process depends on the presence of the nuclear-encoded, chloroplast-localized RNA-binding protein CP29A. In this study, we demonstrate that CP29A undergoes phase separation in vitro and in vivo in a temperature-dependent manner, which is mediated by a prion-like domain (PLD) located between the two RNA recognition motif domains of CP29A. The resulting droplets display liquid-like properties and are found near chloroplast nucleoids. The PLD is required to support chloroplast RNA splicing and translation in cold-treated tissue. Together, our findings suggest that plant chloroplast gene expression is compartmentalized by inducible condensation of CP29A at low temperatures, a mechanism that could play a crucial role in plant cold resistance.
UR - http://www.scopus.com/inward/record.url?scp=85196767008&partnerID=8YFLogxK
U2 - 10.1093/plcell/koae145
DO - 10.1093/plcell/koae145
M3 - Article
C2 - 38723165
AN - SCOPUS:85196767008
SN - 1040-4651
VL - 36
SP - 2851
EP - 2872
JO - Plant Cell
JF - Plant Cell
IS - 8
ER -