A practical guide to photoswitching optoacoustics tomography

Mariia Stankevych, Kanuj Mishra, Vasilis Ntziachristos, Andre C. Stiel

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Photochromic proteins and photoswitching optoacoustics (OA) are a promising combination, that allows OA imaging of even small numbers of cells in whole live animals and thus can facilitate a more wide-spread use of OA in life-science and preclinical research. The concept relies on exploiting the modulation achieved by the photoswitching to discriminate the agents' signal from the non-modulating background. Here we share our analysis approaches that can be readily used on data generated with commercial OA tomography imaging instrumentation allowing—depending on the used photoswitching agent and sample—routine visualizations of as little as several hundreds of transgene labeled cells per imaging volume in the live animal.

Original languageEnglish
Title of host publicationPhotoacoustic Probes for In Vivo Imaging
EditorsJefferson Chan
PublisherAcademic Press Inc.
Pages365-383
Number of pages19
ISBN (Print)9780323855303
DOIs
StatePublished - Jan 2021

Publication series

NameMethods in Enzymology
Volume657
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Bacteriophytochrome
  • Image analysis
  • Imaging
  • Optoacoustic
  • Photoacoustic
  • Photochromic protein
  • Photoswitching
  • Transgene reporter
  • Unmixing

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