@inbook{3573f80177f045d0bf201798b22d85f8,
title = "A practical guide to photoswitching optoacoustics tomography",
abstract = "Photochromic proteins and photoswitching optoacoustics (OA) are a promising combination, that allows OA imaging of even small numbers of cells in whole live animals and thus can facilitate a more wide-spread use of OA in life-science and preclinical research. The concept relies on exploiting the modulation achieved by the photoswitching to discriminate the agents' signal from the non-modulating background. Here we share our analysis approaches that can be readily used on data generated with commercial OA tomography imaging instrumentation allowing—depending on the used photoswitching agent and sample—routine visualizations of as little as several hundreds of transgene labeled cells per imaging volume in the live animal.",
keywords = "Bacteriophytochrome, Image analysis, Imaging, Optoacoustic, Photoacoustic, Photochromic protein, Photoswitching, Transgene reporter, Unmixing",
author = "Mariia Stankevych and Kanuj Mishra and Vasilis Ntziachristos and Stiel, {Andre C.}",
note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jan,
doi = "10.1016/bs.mie.2021.06.031",
language = "English",
isbn = "9780323855303",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "365--383",
editor = "Jefferson Chan",
booktitle = "Photoacoustic Probes for In Vivo Imaging",
}