A photolabile linker for the mild and selective cleavage of enriched biomolecules from solid support

Ronald Orth; Stephan A. Sieber

doi:10.1021/jo901809k

A photolabile linker for the mild and selective cleavage of enriched biomolecules from solid support

Ronald Orth
, Stephan A. Sieber

University of Munich

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

(Chemical Equation Presented) Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.

Original language	English
Pages (from-to)	8476-8479
Number of pages	4
Journal	Journal of Organic Chemistry
Volume	74
Issue number	21
DOIs	https://doi.org/10.1021/jo901809k
State	Published - 6 Nov 2009
Externally published	Yes

Access to Document

10.1021/jo901809k

Cite this

@article{3cbe033f82a94117998414a7178e787d,

title = "A photolabile linker for the mild and selective cleavage of enriched biomolecules from solid support",

abstract = "(Chemical Equation Presented) Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.",

author = "Ronald Orth and Sieber, \{Stephan A.\}",

year = "2009",

month = nov,

day = "6",

doi = "10.1021/jo901809k",

language = "English",

volume = "74",

pages = "8476--8479",

journal = "Journal of Organic Chemistry",

issn = "0022-3263",

publisher = "American Chemical Society",

number = "21",

}

TY - JOUR

T1 - A photolabile linker for the mild and selective cleavage of enriched biomolecules from solid support

AU - Orth, Ronald

AU - Sieber, Stephan A.

PY - 2009/11/6

Y1 - 2009/11/6

N2 - (Chemical Equation Presented) Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.

AB - (Chemical Equation Presented) Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.

UR - https://www.scopus.com/pages/publications/70350733536

U2 - 10.1021/jo901809k

DO - 10.1021/jo901809k

M3 - Article

C2 - 19821566

AN - SCOPUS:70350733536

SN - 0022-3263

VL - 74

SP - 8476

EP - 8479

JO - Journal of Organic Chemistry

JF - Journal of Organic Chemistry

IS - 21

ER -

A photolabile linker for the mild and selective cleavage of enriched biomolecules from solid support

Abstract

Access to Document

Other files and links

Fingerprint

Cite this