A nuclear yeast gene (GCY) encodes a polypeptide with high homology to a vertebrate eye lens protein

Ulrich Oechsner, Viktor Magdolen, Wolfhard Bandlow

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

We describe here the nuclear gene for a yeast protein showing unexpectedly high homology with mammalian aldo/keto reductases as well as with ρ{variant}-crystallin, one of the prominent proteins of the frog eye lens. Although it could be proven that the gene occurs as a single copy in the haploid yeast genome, replacement of the intact by a disrupted, nonfunctional allele led to no obvious phenotype, indicating that the gene is dispensable. The gene was assigned to chromosome XV. It is transcribed in vivo into an mRNA of about 1300 bases with a coding capacity for a protein of 312 amino acids (estimated Mr 35000).

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalFEBS Letters
Volume238
Issue number1
DOIs
StatePublished - 26 Sep 1988
Externally publishedYes

Keywords

  • (Saccharomyces cerevisiae)
  • Aldo/keto reductase family
  • Crystallin
  • Eye lens protein evolution
  • Homology
  • Nucleotide sequence

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