A New Multi-quantum Version of the HBHA(CBCACO)NH Experiment with Enhanced Sensitivity for Partially Deuterated Samples

Ruth M. Gschwind; Horst Kessler; Gerd Gemmecker

doi:10.1006/jmre.1998.1653

A New Multi-quantum Version of the HBHA(CBCACO)NH Experiment with Enhanced Sensitivity for Partially Deuterated Samples

Ruth M. Gschwind, Horst Kessler, Gerd Gemmecker

TUM Emeriti of Excellence

Research output: Contribution to journal › Editorial

3 Scopus citations

Abstract

A new multi-quantum version of the HBHA(CBCACO)NH experiment for partially deuterated protein samples is presented. The method is based on the significant reduction of the proton and carbon relaxation rates due to multi-quantum delays in highly deuterated proteins recently published by our group. The introduction of a multi-quantum period in the coherence transfer pathway of the HBHA(CBCACO)NH experiment yields a dramatic increase of sensitivity - on average 46% with a 75% deuterated sample of the homodimeric 31 kDa E. coli IIA ^Man domain. Additional resolution in the proton dimension can be achieved by a double time shared approach keeping the ¹ H single-quantum period at a minimum.

Original language	English
Pages (from-to)	285-288
Number of pages	4
Journal	Journal of Magnetic Resonance
Volume	137
Issue number	1
DOIs	https://doi.org/10.1006/jmre.1998.1653
State	Published - Mar 1999

Keywords

Multi-quantum
Partially deuterated proteins
Proton side-chain assignment
Triple-resonance NMR

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10.1006/jmre.1998.1653

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title = "A New Multi-quantum Version of the HBHA(CBCACO)NH Experiment with Enhanced Sensitivity for Partially Deuterated Samples",

abstract = " A new multi-quantum version of the HBHA(CBCACO)NH experiment for partially deuterated protein samples is presented. The method is based on the significant reduction of the proton and carbon relaxation rates due to multi-quantum delays in highly deuterated proteins recently published by our group. The introduction of a multi-quantum period in the coherence transfer pathway of the HBHA(CBCACO)NH experiment yields a dramatic increase of sensitivity - on average 46% with a 75% deuterated sample of the homodimeric 31 kDa E. coli IIA Man domain. Additional resolution in the proton dimension can be achieved by a double time shared approach keeping the 1 H single-quantum period at a minimum.",

keywords = "Multi-quantum, Partially deuterated proteins, Proton side-chain assignment, Triple-resonance NMR",

author = "Gschwind, {Ruth M.} and Horst Kessler and Gerd Gemmecker",

note = "Funding Information: We gratefully acknowledge financial support from the Deutsche For-schungsgemeinschaft, the Sonderforschungsbereich 369, the Fonds der che-mischen Industrie, and the Dr.-Ing. Leonhard-Lorenz-Stiftung. We also thank B. Erni and Regula Gutknecht (both at University of Bern) for providing the partially deuterated sample of IIAMan.",

year = "1999",

month = mar,

doi = "10.1006/jmre.1998.1653",

language = "English",

volume = "137",

pages = "285--288",

journal = "Journal of Magnetic Resonance",

issn = "1090-7807",

publisher = "Academic Press Inc.",

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T1 - A New Multi-quantum Version of the HBHA(CBCACO)NH Experiment with Enhanced Sensitivity for Partially Deuterated Samples

AU - Gschwind, Ruth M.

AU - Kessler, Horst

AU - Gemmecker, Gerd

N1 - Funding Information: We gratefully acknowledge financial support from the Deutsche For-schungsgemeinschaft, the Sonderforschungsbereich 369, the Fonds der che-mischen Industrie, and the Dr.-Ing. Leonhard-Lorenz-Stiftung. We also thank B. Erni and Regula Gutknecht (both at University of Bern) for providing the partially deuterated sample of IIAMan.

PY - 1999/3

Y1 - 1999/3

N2 - A new multi-quantum version of the HBHA(CBCACO)NH experiment for partially deuterated protein samples is presented. The method is based on the significant reduction of the proton and carbon relaxation rates due to multi-quantum delays in highly deuterated proteins recently published by our group. The introduction of a multi-quantum period in the coherence transfer pathway of the HBHA(CBCACO)NH experiment yields a dramatic increase of sensitivity - on average 46% with a 75% deuterated sample of the homodimeric 31 kDa E. coli IIA Man domain. Additional resolution in the proton dimension can be achieved by a double time shared approach keeping the 1 H single-quantum period at a minimum.

AB - A new multi-quantum version of the HBHA(CBCACO)NH experiment for partially deuterated protein samples is presented. The method is based on the significant reduction of the proton and carbon relaxation rates due to multi-quantum delays in highly deuterated proteins recently published by our group. The introduction of a multi-quantum period in the coherence transfer pathway of the HBHA(CBCACO)NH experiment yields a dramatic increase of sensitivity - on average 46% with a 75% deuterated sample of the homodimeric 31 kDa E. coli IIA Man domain. Additional resolution in the proton dimension can be achieved by a double time shared approach keeping the 1 H single-quantum period at a minimum.

KW - Multi-quantum

KW - Partially deuterated proteins

KW - Proton side-chain assignment

KW - Triple-resonance NMR

UR - http://www.scopus.com/inward/record.url?scp=0033087066&partnerID=8YFLogxK

U2 - 10.1006/jmre.1998.1653

DO - 10.1006/jmre.1998.1653

M3 - Editorial

C2 - 10053162

AN - SCOPUS:0033087066

SN - 1090-7807

VL - 137

SP - 285

EP - 288

JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

IS - 1

ER -

A New Multi-quantum Version of the HBHA(CBCACO)NH Experiment with Enhanced Sensitivity for Partially Deuterated Samples

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Keywords

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