A multicentre analytical comparison study of inter-reader and inter-assay agreement of four programmed death-ligand 1 immunohistochemistry assays for scoring in triple-negative breast cancer

Aurelia Noske, Johannes U. Ammann, Daniel Christoph Wagner, Carsten Denkert, Annette Lebeau, Peter Sinn, Hans Heinrich Kreipe, Ulrich Sommer, Gustavo Baretton, Katja Steiger, Marion Kiechle, Stefanie Hieke-Schulz, Mike Flores, Wilfried Roth, Wilko Weichert

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Aims: Studies in various cancer types have demonstrated discordance between results from different programmed death-ligand 1 (PD-L1) assays. Here, we compare the reproducibility and analytical concordance of four clinically developed assays for assessing PD-L1-positivity in tumour-infiltrating immune cells in the tumour area (PD-L1-IC-positivity) in triple-negative breast cancer (TNBC). Methods and results: Primary TNBC resection specimens (n = 30) were selected based on their PD-L1-IC-positivity per VENTANA SP142 (<1%: 15 cases; 1–5%: seven cases; >5%: eight cases). Serial histological sections were stained for PD-L1 using VENTANA SP142, VENTANA SP263, DAKO 22C3 and DAKO 28-8. PD-L1-IC-positivity and tumour cell expression (≥1 versus <1%) were scored by trained readers from seven sites using online virtual microscopy. The adjusted mean of PD-L1-IC-positivity for SP263 (7.8%) was significantly higher than those for the other three assays (3.7–4.9%). Differences in adjusted means were statistically significant between SP263 and the other three assays (P < 0.0001) but not between the three remaining assays when excluding SP263 (P = 0.0961–0.6522). Intra-class correlation coefficients revealed moderate-to-strong inter-reader agreement for each assay (0.460–0.805) and poor-to-strong inter-assay agreement for each reader (0.298–0.678) on PD-L1-IC-positivity. Conclusions: In this first multicentre study of different PD-L1 assays in TNBC, we show that PD-L1-IC-positivity for SP142, 22C3 and 28-8 was reproducible and analytically concordant, indicating that these three assays may be analytically interchangeable. The relevance of the higher PD-L1-IC-positivity for SP263 should be further investigated.

Original languageEnglish
Pages (from-to)567-577
Number of pages11
JournalHistopathology
Volume78
Issue number4
DOIs
StatePublished - Mar 2021

Keywords

  • immunohistochemistry
  • inter-assay agreement
  • inter-reader agreement
  • programmed death-ligand 1
  • triple-negative breast cancer

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