TY - JOUR
T1 - A multicentre analytical comparison study of inter-reader and inter-assay agreement of four programmed death-ligand 1 immunohistochemistry assays for scoring in triple-negative breast cancer
AU - Noske, Aurelia
AU - Ammann, Johannes U.
AU - Wagner, Daniel Christoph
AU - Denkert, Carsten
AU - Lebeau, Annette
AU - Sinn, Peter
AU - Kreipe, Hans Heinrich
AU - Sommer, Ulrich
AU - Baretton, Gustavo
AU - Steiger, Katja
AU - Kiechle, Marion
AU - Hieke-Schulz, Stefanie
AU - Flores, Mike
AU - Roth, Wilfried
AU - Weichert, Wilko
N1 - Publisher Copyright:
© 2020 The Authors. Histopathology published by John Wiley & Sons Ltd
PY - 2021/3
Y1 - 2021/3
N2 - Aims: Studies in various cancer types have demonstrated discordance between results from different programmed death-ligand 1 (PD-L1) assays. Here, we compare the reproducibility and analytical concordance of four clinically developed assays for assessing PD-L1-positivity in tumour-infiltrating immune cells in the tumour area (PD-L1-IC-positivity) in triple-negative breast cancer (TNBC). Methods and results: Primary TNBC resection specimens (n = 30) were selected based on their PD-L1-IC-positivity per VENTANA SP142 (<1%: 15 cases; 1–5%: seven cases; >5%: eight cases). Serial histological sections were stained for PD-L1 using VENTANA SP142, VENTANA SP263, DAKO 22C3 and DAKO 28-8. PD-L1-IC-positivity and tumour cell expression (≥1 versus <1%) were scored by trained readers from seven sites using online virtual microscopy. The adjusted mean of PD-L1-IC-positivity for SP263 (7.8%) was significantly higher than those for the other three assays (3.7–4.9%). Differences in adjusted means were statistically significant between SP263 and the other three assays (P < 0.0001) but not between the three remaining assays when excluding SP263 (P = 0.0961–0.6522). Intra-class correlation coefficients revealed moderate-to-strong inter-reader agreement for each assay (0.460–0.805) and poor-to-strong inter-assay agreement for each reader (0.298–0.678) on PD-L1-IC-positivity. Conclusions: In this first multicentre study of different PD-L1 assays in TNBC, we show that PD-L1-IC-positivity for SP142, 22C3 and 28-8 was reproducible and analytically concordant, indicating that these three assays may be analytically interchangeable. The relevance of the higher PD-L1-IC-positivity for SP263 should be further investigated.
AB - Aims: Studies in various cancer types have demonstrated discordance between results from different programmed death-ligand 1 (PD-L1) assays. Here, we compare the reproducibility and analytical concordance of four clinically developed assays for assessing PD-L1-positivity in tumour-infiltrating immune cells in the tumour area (PD-L1-IC-positivity) in triple-negative breast cancer (TNBC). Methods and results: Primary TNBC resection specimens (n = 30) were selected based on their PD-L1-IC-positivity per VENTANA SP142 (<1%: 15 cases; 1–5%: seven cases; >5%: eight cases). Serial histological sections were stained for PD-L1 using VENTANA SP142, VENTANA SP263, DAKO 22C3 and DAKO 28-8. PD-L1-IC-positivity and tumour cell expression (≥1 versus <1%) were scored by trained readers from seven sites using online virtual microscopy. The adjusted mean of PD-L1-IC-positivity for SP263 (7.8%) was significantly higher than those for the other three assays (3.7–4.9%). Differences in adjusted means were statistically significant between SP263 and the other three assays (P < 0.0001) but not between the three remaining assays when excluding SP263 (P = 0.0961–0.6522). Intra-class correlation coefficients revealed moderate-to-strong inter-reader agreement for each assay (0.460–0.805) and poor-to-strong inter-assay agreement for each reader (0.298–0.678) on PD-L1-IC-positivity. Conclusions: In this first multicentre study of different PD-L1 assays in TNBC, we show that PD-L1-IC-positivity for SP142, 22C3 and 28-8 was reproducible and analytically concordant, indicating that these three assays may be analytically interchangeable. The relevance of the higher PD-L1-IC-positivity for SP263 should be further investigated.
KW - immunohistochemistry
KW - inter-assay agreement
KW - inter-reader agreement
KW - programmed death-ligand 1
KW - triple-negative breast cancer
UR - http://www.scopus.com/inward/record.url?scp=85096868946&partnerID=8YFLogxK
U2 - 10.1111/his.14254
DO - 10.1111/his.14254
M3 - Article
C2 - 32936950
AN - SCOPUS:85096868946
SN - 0309-0167
VL - 78
SP - 567
EP - 577
JO - Histopathology
JF - Histopathology
IS - 4
ER -