Abstract
Large quantities of Toxoplasma gondii were cultivated in human larynx carcinoma cells (H.Ep 2, heteroploid). Virulent Toxoplasma gondii, strain RH, which were obtained from the peritoneal exsudate of mice 2 days after infection served as inoculum. As culture medium for the cells and for growth medium, Minimum Essential Medium (MEM) supplemented by 5% foetal calf serum and 200,000 IE/1 Penicillin plus 20 mg/1 Sulmycin was used. 2--3 days after inoculation, 150--200 times the amount of the inoculated Toxoplasmas could be harvested in one or several harvests.
| Original language | English |
|---|---|
| Pages (from-to) | 432-434 |
| Number of pages | 3 |
| Journal | Tropenmedizin und Parasitologie |
| Volume | 29 |
| Issue number | 4 |
| State | Published - Dec 1978 |
