TY - JOUR
T1 - A method for determining the mutual diffusion coefficient of molecular solutes based on surface plasmon resonance sensing
AU - Loureiro, F. C.C.L.
AU - Barreto Neto, A. G.S.
AU - Moreira, C. S.
AU - Lima, A. M.N.
AU - Neff, H.
N1 - Funding Information:
The authors would like to thank CNPq and CETENE/LINCS for the research grant and financial support.
PY - 2011/6/20
Y1 - 2011/6/20
N2 - An experimental method, combining surface plasmon resonance sensing and microfluidics, to determine the mutual diffusion coefficient of molecular solutes, as ethanol and bovine serum albumin, is presented. Representative refractive index variations of analyte samples over time, and associated dynamic solute concentration profiles, respectively, have been employed to access molecular transport parameters. From both, Fick's diffusion length and Taylor's pulse dispersion methods, solute and solvent mutual diffusion coefficients for diluted ethanol and concentrated protein aqueous solutions have been obtained. Additionally, the dynamic behavior and geometry effects of molecular transport have been exploited using finite element simulations for the 3-dimensional case and confirmed experimentally. The numerical simulation also addresses the influence of temperature effects.
AB - An experimental method, combining surface plasmon resonance sensing and microfluidics, to determine the mutual diffusion coefficient of molecular solutes, as ethanol and bovine serum albumin, is presented. Representative refractive index variations of analyte samples over time, and associated dynamic solute concentration profiles, respectively, have been employed to access molecular transport parameters. From both, Fick's diffusion length and Taylor's pulse dispersion methods, solute and solvent mutual diffusion coefficients for diluted ethanol and concentrated protein aqueous solutions have been obtained. Additionally, the dynamic behavior and geometry effects of molecular transport have been exploited using finite element simulations for the 3-dimensional case and confirmed experimentally. The numerical simulation also addresses the influence of temperature effects.
KW - Diffusivity
KW - Finite element simulation
KW - Microfluidic
KW - Molecular transport
KW - Surface plasmon resonance sensing
UR - http://www.scopus.com/inward/record.url?scp=77956061607&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2010.02.023
DO - 10.1016/j.snb.2010.02.023
M3 - Article
AN - SCOPUS:77956061607
SN - 0925-4005
VL - 154
SP - 129
EP - 136
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 2
ER -