A large size-selective DNA nanopore with sensing applications

Rasmus P. Thomsen, Mette Galsgaard Malle, Anders Hauge Okholm, Swati Krishnan, Søren S.R. Bohr, Rasmus Schøler Sørensen, Oliver Ries, Stefan Vogel, Friedrich C. Simmel, Nikos S. Hatzakis, Jørgen Kjems

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

Transmembrane nanostructures like ion channels and transporters perform key biological functions by controlling flow of molecules across lipid bilayers. Much work has gone into engineering artificial nanopores and applications in selective gating of molecules, label-free detection/sensing of biomolecules and DNA sequencing have shown promise. Here, we use DNA origami to create a synthetic 9 nm wide DNA nanopore, controlled by programmable, lipidated flaps and equipped with a size-selective gating system for the translocation of macromolecules. Successful assembly and insertion of the nanopore into lipid bilayers are validated by transmission electron microscopy (TEM), while selective translocation of cargo and the pore mechanosensitivity are studied using optical methods, including single-molecule, total internal reflection fluorescence (TIRF) microscopy. Size-specific cargo translocation and oligonucleotide-triggered opening of the pore are demonstrated showing that the DNA nanopore can function as a real-time detection system for external signals, offering potential for a variety of highly parallelized sensing applications.

Original languageEnglish
Article number5655
JournalNature Communications
Volume10
Issue number1
DOIs
StatePublished - 1 Dec 2019

Fingerprint

Dive into the research topics of 'A large size-selective DNA nanopore with sensing applications'. Together they form a unique fingerprint.

Cite this