A general method for gene isolation in tagging approaches: Amplification of insertion mutagenised sites (AIMS)

Monika Frey; Cornelia Stettner; Alfons Gierl

doi:10.1046/j.1365-313X.1998.00091.x

A general method for gene isolation in tagging approaches: Amplification of insertion mutagenised sites (AIMS)

Monika Frey, Cornelia Stettner, Alfons Gierl

Life Sciences

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T-DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator element.

Original language	English
Pages (from-to)	717-721
Number of pages	5
Journal	Plant Journal
Volume	13
Issue number	5
DOIs	https://doi.org/10.1046/j.1365-313X.1998.00091.x
State	Published - 1998

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10.1046/j.1365-313X.1998.00091.x

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abstract = "A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T-DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator element.",

author = "Monika Frey and Cornelia Stettner and Alfons Gierl",

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AU - Gierl, Alfons

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AB - A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T-DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator element.

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U2 - 10.1046/j.1365-313X.1998.00091.x

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A general method for gene isolation in tagging approaches: Amplification of insertion mutagenised sites (AIMS)

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