A general enhancement scheme in heteronuclear multidimensional NMR employing pulsed field gradients

J. Schleucher, M. Schwendinger, M. Sattler, P. Schmidt, O. Schedletzky, S. J. Glaser, O. W. Sørensen, C. Griesinger

Research output: Contribution to journalArticlepeer-review

755 Scopus citations

Abstract

General pulse sequence elements that achieve sensitivity-enhanced coherence transfer from a heteronucleus to protons of arbitrary multiplicity are introduced. The building blocks are derived from the sensitivity-enhancement scheme introduced by Cavanagh et al. ((1991) J. Magn. Reson., 91, 429-436), which was used in conjunction with gradient coherence selection by Kay et al. ((1992) J. Am. Chem. Soc., 114, 10663-10665), as well as from a multiple-pulse sequence effecting a heteronuclear planar coupling Hamiltonian. The building blocks are incorporated into heteronuclear correlation experiments, in conjunction with coherence selection by the formation of a heteronuclear gradient echo. This allows for efficient water suppression without the need for water presaturation. The methods are demonstrated in HSQC-type experiments on a sample of a decapeptide in H2O. The novel pulse sequence elements can be incorporated into multidimensional experiments.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalJournal of Biomolecular NMR
Volume4
Issue number2
DOIs
StatePublished - Mar 1994
Externally publishedYes

Keywords

  • B-gradients
  • Coherence transfer
  • HSQC
  • Heteronuclear Hartmann-Hahn spectroscopy
  • Heteronuclear NMR
  • Planar TOCSY
  • Sensitivity enhancement
  • Solvent suppression

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