A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria

Maria Knauer; Natalia P. Ivleva; Reinhard Niessner; Christoph Haisch

doi:10.1007/s00216-011-5398-0

A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria

Maria Knauer, Natalia P. Ivleva, Reinhard Niessner, Christoph Haisch

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

We present an immunoassay microarray flow-through system for the surface-enhanced Raman scattering (SERS) analysis of bacteria. The system has been constructed to support and automatize the nondestructive in situ analysis of different microorganisms in aqueous environment. After the immobilization of the desired antibodies to an activated PEG-coated surface, the chip is placed into the flow cell which is then flushed with the contaminated sample. Finally, colloidal metal nanoparticles are added and the cells are detected label-free by SERS. Here, we introduce the successful imaging of single microorganisms in the flow cell as well as the quantification of microorganisms in water by SERS mapping with a linear range between 4.3∈×∈10³ to 4.3∈×∈10⁵ cells/mL. The method has potential for routine application, e.g. for drinking water control.

Original language	English
Pages (from-to)	2663-2667
Number of pages	5
Journal	Analytical and Bioanalytical Chemistry
Volume	402
Issue number	8
DOIs	https://doi.org/10.1007/s00216-011-5398-0
State	Published - Mar 2012

Keywords

Flow cell
Microarray
Microorganisms
SERS

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10.1007/s00216-011-5398-0

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abstract = "We present an immunoassay microarray flow-through system for the surface-enhanced Raman scattering (SERS) analysis of bacteria. The system has been constructed to support and automatize the nondestructive in situ analysis of different microorganisms in aqueous environment. After the immobilization of the desired antibodies to an activated PEG-coated surface, the chip is placed into the flow cell which is then flushed with the contaminated sample. Finally, colloidal metal nanoparticles are added and the cells are detected label-free by SERS. Here, we introduce the successful imaging of single microorganisms in the flow cell as well as the quantification of microorganisms in water by SERS mapping with a linear range between 4.3∈×∈103 to 4.3∈×∈105 cells/mL. The method has potential for routine application, e.g. for drinking water control.",

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AU - Haisch, Christoph

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AB - We present an immunoassay microarray flow-through system for the surface-enhanced Raman scattering (SERS) analysis of bacteria. The system has been constructed to support and automatize the nondestructive in situ analysis of different microorganisms in aqueous environment. After the immobilization of the desired antibodies to an activated PEG-coated surface, the chip is placed into the flow cell which is then flushed with the contaminated sample. Finally, colloidal metal nanoparticles are added and the cells are detected label-free by SERS. Here, we introduce the successful imaging of single microorganisms in the flow cell as well as the quantification of microorganisms in water by SERS mapping with a linear range between 4.3∈×∈103 to 4.3∈×∈105 cells/mL. The method has potential for routine application, e.g. for drinking water control.

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A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria

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Keywords

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