A facile direct nucleophilic synthesis of O-(2-[18F]fluoroethyl) -L-tyrosine ([18F]FET) without HPLC purification

Olga Fedorova, Olga Kuznetsova, Maria Stepanova, Victor Maleev, Yuri Belokon, Hans Juergen Wester, Raisa Krasikova

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Due to favourable in vivo characteristics, its high specificity and the longer half-life of 18F (109.8 min) allowing for remote-site delivery, O-(2-[18F]fluoroethyl)-l-tyrosine ([18F]FET) has gained increased importance for molecular imaging of cerebral tumors. Consequently, the development of simple and efficient production strategies for [18F]FET could be an important step to further improve the cost-effective availability of [18F]FET in the clinical environment. In the present study [18F]FET was synthesized via direct nucleophilic synthesis using an earlier developed chiral precursor, the NiII complex of an alkylated (S)-tyrosine Schiff base, Ni-(S)-BPB-(S)-Tyr-OCH 2CH2OTs. The purification method has been developed via solid phase extraction thereby omitting cumbersome HPLC purification. The suggested SPE purification using combination of reverse phase and strong cation exchange cartridges provided [18F]FET in high chemical, radiochemical and enantiomeric purity and 35 % radiochemical yield (decay-corrected, 45 min synthesis time). The method was successfully automated using a commercially available synthesis module, Scintomics Hotboxone. Based on the current results, the proposed production route appears to be well suited for transfer into an automated cassette-type radiosynthesizers without using HPLC.

Original languageEnglish
Pages (from-to)505-512
Number of pages8
JournalJournal of Radioanalytical and Nuclear Chemistry
Issue number2
StatePublished - Aug 2014


  • Fluorine-18
  • PET
  • SPE purification


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