A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Julian Grünewald; Ronghao Zhou; Caleb A. Lareau; Sara P. Garcia; Sowmya Iyer; Bret R. Miller; Lukas M. Langner; Jonathan Y. Hsu; Martin J. Aryee; J. Keith Joung

doi:10.1038/s41587-020-0535-y

A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Julian Grünewald, Ronghao Zhou, Caleb A. Lareau, Sara P. Garcia, Sowmya Iyer, Bret R. Miller, Lukas M. Langner, Jonathan Y. Hsu, Martin J. Aryee, J. Keith Joung

Research output: Contribution to journal › Article › peer-review

202 Scopus citations

Abstract

Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.

Original language	English
Pages (from-to)	861-864
Number of pages	4
Journal	Nature Biotechnology
Volume	38
Issue number	7
DOIs	https://doi.org/10.1038/s41587-020-0535-y
State	Published - 1 Jul 2020
Externally published	Yes

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title = "A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing",

abstract = "Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.",

author = "Julian Gr{\"u}newald and Ronghao Zhou and Lareau, {Caleb A.} and Garcia, {Sara P.} and Sowmya Iyer and Miller, {Bret R.} and Langner, {Lukas M.} and Hsu, {Jonathan Y.} and Aryee, {Martin J.} and Joung, {J. Keith}",

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AU - Grünewald, Julian

AU - Zhou, Ronghao

AU - Lareau, Caleb A.

AU - Garcia, Sara P.

AU - Iyer, Sowmya

AU - Miller, Bret R.

AU - Langner, Lukas M.

AU - Hsu, Jonathan Y.

AU - Aryee, Martin J.

AU - Joung, J. Keith

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.

AB - Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.

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VL - 38

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JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 7

ER -

A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

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