TY - JOUR
T1 - A critical comparison of cell-based sensor systems for the detection of Cr(VI) in aquatic environment
AU - Bohrn, Ulrich
AU - Mucha, Andreas
AU - Werner, Carl Frederik
AU - Trattner, Barbara
AU - Bäcker, Matthias
AU - Krumbe, Christoph
AU - Schienle, Meinrad
AU - Stütz, Evamaria
AU - Schmitt-Landsiedel, Doris
AU - Fleischer, Maximilian
AU - Wagner, Patrick
AU - Schöning, Michael J.
PY - 2013
Y1 - 2013
N2 - The toxicity of chromium ions was investigated using mammalian cell cultures on impedance sensors as well as physiological in vitro sensor systems. The performance of commercially available systems like the 2500 Analyzing System (Bionas), xCELLigence (Roche) and Cytosensor Microphysiometer (Molecular Devices) was compared with a novel CMOS-based impedance-to-frequency converter device. Cell-based sensor systems are shown to be powerful tools to detect Cr(VI) pollutions within several hours in the range of multinational drinking water regulations. The ability to distinguish between toxic Cr(VI) and non-toxic Cr(III) species is one advantage of these integral sensor systems. Impedance only devices are not sufficient for the fast detection of toxic chromium species as rapid cellular changes occur only in the respiration system and the cell physiology.
AB - The toxicity of chromium ions was investigated using mammalian cell cultures on impedance sensors as well as physiological in vitro sensor systems. The performance of commercially available systems like the 2500 Analyzing System (Bionas), xCELLigence (Roche) and Cytosensor Microphysiometer (Molecular Devices) was compared with a novel CMOS-based impedance-to-frequency converter device. Cell-based sensor systems are shown to be powerful tools to detect Cr(VI) pollutions within several hours in the range of multinational drinking water regulations. The ability to distinguish between toxic Cr(VI) and non-toxic Cr(III) species is one advantage of these integral sensor systems. Impedance only devices are not sufficient for the fast detection of toxic chromium species as rapid cellular changes occur only in the respiration system and the cell physiology.
KW - Acidification
KW - Cell-based biosensor
KW - Chromium
KW - Impedance
KW - Respiration
KW - V79
UR - http://www.scopus.com/inward/record.url?scp=84875488978&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2013.02.105
DO - 10.1016/j.snb.2013.02.105
M3 - Article
AN - SCOPUS:84875488978
SN - 0925-4005
VL - 182
SP - 58
EP - 65
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -