A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil

Stephan Schulz; Alfredo Peréz-de-Mora; Marion Engel; Jean Charles Munch; Michael Schloter

doi:10.1016/j.mimet.2010.01.005

A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil

Stephan Schulz
, Alfredo Peréz-de-Mora
, Marion Engel
, Jean Charles Munch
, Michael Schloter

Life Sciences

Technical University of Munich
Helmholtz Zentrum München German Research Center for Environmental Health

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content.

Original language	English
Pages (from-to)	295-298
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	80
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2010.01.005
State	Published - Mar 2010

Keywords

Alkane monooxygenase gene alkB
MPN-PCR
Quantitative PCR
Real-time PCR

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10.1016/j.mimet.2010.01.005

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title = "A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil",

abstract = "The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content.",

keywords = "Alkane monooxygenase gene alkB, MPN-PCR, Quantitative PCR, Real-time PCR",

author = "Stephan Schulz and Alfredo Per{\'e}z-de-Mora and Marion Engel and Munch, \{Jean Charles\} and Michael Schloter",

year = "2010",

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doi = "10.1016/j.mimet.2010.01.005",

language = "English",

volume = "80",

pages = "295--298",

journal = "Journal of Microbiological Methods",

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A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil. / Schulz, Stephan; Peréz-de-Mora, Alfredo; Engel, Marion et al.
In: Journal of Microbiological Methods, Vol. 80, No. 3, 03.2010, p. 295-298.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil

AU - Schulz, Stephan

AU - Peréz-de-Mora, Alfredo

AU - Engel, Marion

AU - Munch, Jean Charles

AU - Schloter, Michael

PY - 2010/3

Y1 - 2010/3

N2 - The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content.

AB - The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content.

KW - Alkane monooxygenase gene alkB

KW - MPN-PCR

KW - Quantitative PCR

KW - Real-time PCR

UR - https://www.scopus.com/pages/publications/77952295773

U2 - 10.1016/j.mimet.2010.01.005

DO - 10.1016/j.mimet.2010.01.005

M3 - Article

C2 - 20079768

AN - SCOPUS:77952295773

SN - 0167-7012

VL - 80

SP - 295

EP - 298

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil

Abstract

Keywords

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