A 16S rRNA Targeted Oligonucleotide Probe for the Differentiation of Staphylococcus haemolyticus from S. warneri and S. hominis

B. Druel; M. Weizenegger; W. Ludwig; J. Freney; K. H. Schleifer; J. Fleurette

doi:10.1016/S0723-2020(11)80113-X

A 16S rRNA Targeted Oligonucleotide Probe for the Differentiation of Staphylococcus haemolyticus from S. warneri and S. hominis

B. Druel
, M. Weizenegger
, W. Ludwig
, J. Freney
, K. H. Schleifer
, J. Fleurette

TUM Emeriti of Excellence

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Sequencing and comparison of 16S rRNA genes of Staphylococcus haemolyticus, S. hominis and S. carnosus allowed the development of an oligonucleotide probe that is complementary to a region of S. haemolyticus 16S rRNA and allows the differentiation of this species from the other two. After hybridization with 27 staphylococcal reference strains and 63 clinical strains, we found this probe useful as an alternative to the ID 32 Staph gallery (bioMérieux) in the case of an identification problem between S. haemolyticus and S. hominis or S. warneri. The oligonucleotide probe was used for the characterization of two misidentified strains.

Original language	English
Pages (from-to)	535-537
Number of pages	3
Journal	Systematic and Applied Microbiology
Volume	15
Issue number	4
DOIs	https://doi.org/10.1016/S0723-2020(11)80113-X
State	Published - 1992

Keywords

16S rRNA
Coagulase-negative staphylococci
Oligonucleotide probe

Access to Document

10.1016/S0723-2020(11)80113-X

Cite this

@article{cc3db90ad84a4bbab39c50daaf175afa,

title = "A 16S rRNA Targeted Oligonucleotide Probe for the Differentiation of Staphylococcus haemolyticus from S. warneri and S. hominis",

abstract = "Sequencing and comparison of 16S rRNA genes of Staphylococcus haemolyticus, S. hominis and S. carnosus allowed the development of an oligonucleotide probe that is complementary to a region of S. haemolyticus 16S rRNA and allows the differentiation of this species from the other two. After hybridization with 27 staphylococcal reference strains and 63 clinical strains, we found this probe useful as an alternative to the ID 32 Staph gallery (bioM{\'e}rieux) in the case of an identification problem between S. haemolyticus and S. hominis or S. warneri. The oligonucleotide probe was used for the characterization of two misidentified strains.",

keywords = "16S rRNA, Coagulase-negative staphylococci, Oligonucleotide probe",

author = "B. Druel and M. Weizenegger and W. Ludwig and J. Freney and Schleifer, \{K. H.\} and J. Fleurette",

year = "1992",

doi = "10.1016/S0723-2020(11)80113-X",

language = "English",

volume = "15",

pages = "535--537",

journal = "Systematic and Applied Microbiology",

issn = "0723-2020",

publisher = "Elsevier GmbH",

number = "4",

}

TY - JOUR

T1 - A 16S rRNA Targeted Oligonucleotide Probe for the Differentiation of Staphylococcus haemolyticus from S. warneri and S. hominis

AU - Druel, B.

AU - Weizenegger, M.

AU - Ludwig, W.

AU - Freney, J.

AU - Schleifer, K. H.

AU - Fleurette, J.

PY - 1992

Y1 - 1992

N2 - Sequencing and comparison of 16S rRNA genes of Staphylococcus haemolyticus, S. hominis and S. carnosus allowed the development of an oligonucleotide probe that is complementary to a region of S. haemolyticus 16S rRNA and allows the differentiation of this species from the other two. After hybridization with 27 staphylococcal reference strains and 63 clinical strains, we found this probe useful as an alternative to the ID 32 Staph gallery (bioMérieux) in the case of an identification problem between S. haemolyticus and S. hominis or S. warneri. The oligonucleotide probe was used for the characterization of two misidentified strains.

AB - Sequencing and comparison of 16S rRNA genes of Staphylococcus haemolyticus, S. hominis and S. carnosus allowed the development of an oligonucleotide probe that is complementary to a region of S. haemolyticus 16S rRNA and allows the differentiation of this species from the other two. After hybridization with 27 staphylococcal reference strains and 63 clinical strains, we found this probe useful as an alternative to the ID 32 Staph gallery (bioMérieux) in the case of an identification problem between S. haemolyticus and S. hominis or S. warneri. The oligonucleotide probe was used for the characterization of two misidentified strains.

KW - 16S rRNA

KW - Coagulase-negative staphylococci

KW - Oligonucleotide probe

UR - https://www.scopus.com/pages/publications/0027050770

U2 - 10.1016/S0723-2020(11)80113-X

DO - 10.1016/S0723-2020(11)80113-X

M3 - Article

AN - SCOPUS:0027050770

SN - 0723-2020

VL - 15

SP - 535

EP - 537

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

IS - 4

ER -

A 16S rRNA Targeted Oligonucleotide Probe for the Differentiation of Staphylococcus haemolyticus from S. warneri and S. hominis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this