[68Ga]Pentixafor-PET/MRI for the detection of Chemokine receptor 4 expression in atherosclerotic plaques

Purpose: The expression of chemokine receptor type 4 (CXCR4) was found co-localized with macrophages on the atherosclerotic vessel wall and participated in the initial emigration of leukocytes. Gallium-68 [⁶⁸Ga]Pentixafor has recently been introduced for the imaging of atherosclerosis by targeting CXCR4. We sought to evaluate human atherosclerotic lesions using [⁶⁸Ga]Pentixafor PET/MRI. Methods: Thirty-eight oncology patients underwent [⁶⁸Ga]Pentixafor PET/MR imaging at baseline. Maximum standardized uptake values (SUV_max) were derived from hot lesions in seven arterial segments and target-to-blood ratios (TBR) were calculated. ANOVA post-hoc and paired t test were performed for statistical comparison, Spearman’s correlation coefficient between uptake ratios and cardiovascular risk factors were assessed. The reproducibility of [⁶⁸Ga]Pentixafor PET/MRI was assessed in seven patients with a follow-up exanimation by Pearson’s regression and Bland–Altman plots analysis. Results: Thirty-four of 38 patients showed 611 focal [⁶⁸Ga]Pentixafor uptake that followed the contours of the large arteries. Both prevalence and mean TBR_max were highest in the descending aorta. There were significantly higher TBR values found in men (1.9 ± 0.3) as compared to women (1.7 ± 0.2; p < 0.05). Patients with mean TBR_max > 1.7 showed a significantly higher incidence of diabetes, hypertension hypercholesterolemia and history of cardiovascular disease than patients with mean TBR_max ≤ 1.7. [⁶⁸Ga]Pentixafor uptake showed a good reproducibility (r = 0.6, p < 0.01), and there was no difference between the mean TBR_max values of plaque lesions (TBR_baseline1.8 ± 0.3 vs TBR_follow-up1.8 ± 0.3) (p = 0.9). Conclusion: Patients with high arterial uptake showed increased incidence of cardiovascular risk factors, suggesting a potential role of [⁶⁸Ga]Pentixafor in characterization of atherosclerosis.