@inbook{d66d83a2784a4a78bf808f57d2b12936,
title = "3D cancer migration assay with Schwann cells",
abstract = "In pancreatic cancer, neural invasion is one of the most common paths of cancer dissemination. Classically, cancer cells actively invade nerves and cause local recurrence and pain. Three-dimensional (3D) neural migration assay has become a standard tool for scientists to study neural invasion by confronting the involved cell types. This protocol introduces Schwann cells, i.e., the most prevalent cell type in peripheral nerves, in a novel heterotypic, glia-cancer-neuron, 3D migration assay for assessing their relevance in the early pathogenesis of neural invasion. Particularly, this assay allows the monitoring of the early Schwann cell migratory activity.",
keywords = "3D migration assay, Dorsal root ganglia/DRG, Neural invasion, Pancreatic cancer, Schwann cell carcinotropism, Schwann cells",
author = "Laura Fangmann and Steffen Teller and Pavel Stupakov and Helmut Friess and Ceyhan, {G{\"u}ralp O.} and Demir, {Ihsan Ekin}",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC.",
year = "2018",
doi = "10.1007/978-1-4939-7649-2_20",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "317--325",
booktitle = "Methods in Molecular Biology",
}
