3D cancer migration assay with Schwann cells

Laura Fangmann, Steffen Teller, Pavel Stupakov, Helmut Friess, Güralp O. Ceyhan, Ihsan Ekin Demir

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Scopus citations

Abstract

In pancreatic cancer, neural invasion is one of the most common paths of cancer dissemination. Classically, cancer cells actively invade nerves and cause local recurrence and pain. Three-dimensional (3D) neural migration assay has become a standard tool for scientists to study neural invasion by confronting the involved cell types. This protocol introduces Schwann cells, i.e., the most prevalent cell type in peripheral nerves, in a novel heterotypic, glia-cancer-neuron, 3D migration assay for assessing their relevance in the early pathogenesis of neural invasion. Particularly, this assay allows the monitoring of the early Schwann cell migratory activity.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages317-325
Number of pages9
DOIs
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1739
ISSN (Print)1064-3745

Keywords

  • 3D migration assay
  • Dorsal root ganglia/DRG
  • Neural invasion
  • Pancreatic cancer
  • Schwann cell carcinotropism
  • Schwann cells

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