2,5-dimethyl-4-hydroxy-3 [2H]-furanone 6'O-malonyl-β-D-glucopyranoside in strawberry fruits

2,5-Dimethyl-4-hydroxy 3[2H]-furanone 6'-O-malonyl-β-D-glucopyranoside was isolated from a glycosidic extract of strawberry fruit (Fragaria X ananassa, cv. Senga Sengana) by means of countercurrent chromatography and reverse-phase HPLC. Identification was achieved by comparison of chromatographic and ¹H, ¹³C and 2D-NMR, as well as mass spectral data with those of the synthesized reference compound. In ripe strawberry fruit, a 1:1 ratio of the malonylated glucoside to its deacylated glucoconjugate was determined by on-line liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry.