TY - JOUR
T1 - 1Sl(1B) Chromosome substitution in Chinese Spring wheat promotes starch granule development and starch biosynthesis
AU - Cao, Min
AU - Chen, Guanxing
AU - Wang, Chang
AU - Zhen, Shoumin
AU - Li, Xiaohui
AU - Zhang, Wenying
AU - Zeller, F. J.
AU - Hsam, S. L.K.
AU - Hu, Yingkao
AU - Yan, Yueming
PY - 2015
Y1 - 2015
N2 - The common wheat variety Chinese Spring (CS) chromosome substitution line CS-1Sl(1B) was used in this study, in which the 1B chromosome in CS (Triticum aestivum L., 2n≤6x≤42, AABBDD) was substituted by the 1Sl from Aegilops longissima (2n≤2x≤14, SlSl). The results showed that the substitution of 1B in CS by 1Sl chromosome could significantly increase amylopectin and total starch contents. The dynamic changes in starch granules during grain development in CS and CS-1Sl(1B) demonstrated that the substitution line possessed higher amount of A-type starch granules and greater diameter of both A- and B-granules. qRT-PCR revealed that some key genes involved in starch biosynthesis, such as starch synthases (SSI, SSII and SSIII), starch branching enzymes (SBE IIa and SBE IIb) and granule-bound starch synthase (GBSS I), displayed higher transcript levels of mRNA expressions during grain development in CS-1Sl(1B). Our results indicate that the substituted 1Sl chromosome carries important genes that influence starch granule development and starch biosynthesis, which may be used as potential gene resources for improvement of wheat starch quality.
AB - The common wheat variety Chinese Spring (CS) chromosome substitution line CS-1Sl(1B) was used in this study, in which the 1B chromosome in CS (Triticum aestivum L., 2n≤6x≤42, AABBDD) was substituted by the 1Sl from Aegilops longissima (2n≤2x≤14, SlSl). The results showed that the substitution of 1B in CS by 1Sl chromosome could significantly increase amylopectin and total starch contents. The dynamic changes in starch granules during grain development in CS and CS-1Sl(1B) demonstrated that the substitution line possessed higher amount of A-type starch granules and greater diameter of both A- and B-granules. qRT-PCR revealed that some key genes involved in starch biosynthesis, such as starch synthases (SSI, SSII and SSIII), starch branching enzymes (SBE IIa and SBE IIb) and granule-bound starch synthase (GBSS I), displayed higher transcript levels of mRNA expressions during grain development in CS-1Sl(1B). Our results indicate that the substituted 1Sl chromosome carries important genes that influence starch granule development and starch biosynthesis, which may be used as potential gene resources for improvement of wheat starch quality.
KW - 1S genome
KW - qRT-PCR
KW - starch biosynthesis
KW - starch granules
KW - wheat substitution line
UR - http://www.scopus.com/inward/record.url?scp=84940920144&partnerID=8YFLogxK
U2 - 10.1071/CP14256
DO - 10.1071/CP14256
M3 - Article
AN - SCOPUS:84940920144
SN - 1836-0947
VL - 66
SP - 894
EP - 903
JO - Crop and Pasture Science
JF - Crop and Pasture Science
IS - 9
ER -