TY - JOUR
T1 - Value-Added Squalene in Single-Cell Oil Produced with Cutaneotrichosporon oleaginosus for Food Applications
AU - Stellner, N. I.
AU - Rerop, Z. S.
AU - Kyselka, Jan
AU - Alishevich, Katsiaryna
AU - Beneš, Radek
AU - Filip, Vladimír
AU - Celik, Gülnaz
AU - Haack, Martina
AU - Ringel, Marion
AU - Masri, Mahmoud
AU - Brück, Thomas
N1 - Publisher Copyright:
© 2023 American Chemical Society. All rights reserved.
PY - 2023/6/7
Y1 - 2023/6/7
N2 - Single-cell oil (SCO) produced by oleaginous microorganisms is potentially a more land-efficient and sustainable alternative to vegetable oil. The cost of SCO production can be reduced by value-added co-products like squalene, a highly relevant compound for the food, cosmetic, and pharmaceutical industry. For the first time, squalene in the oleaginous yeast Cutaneotrichosporon oleaginosus was analyzed, reaching 172.95 ± 61.31 mg/100 g oil in a lab-scale bioreactor. Using the squalene monooxygenase inhibitor terbinafine, cellular squalene was significantly increased to 2169 ± 262 mg/100 g SCO, while the yeast remained highly oleaginous. Further, SCO from a 1000 L scale production was chemically refined. The squalene content in the deodorizer distillate (DD) was found to be higher than that in DD from typical vegetable oils. Overall, this study demonstrates squalene as a value-added compound in SCO from C. oleaginosus for application in food and cosmetics without the use of genetic modifications.
AB - Single-cell oil (SCO) produced by oleaginous microorganisms is potentially a more land-efficient and sustainable alternative to vegetable oil. The cost of SCO production can be reduced by value-added co-products like squalene, a highly relevant compound for the food, cosmetic, and pharmaceutical industry. For the first time, squalene in the oleaginous yeast Cutaneotrichosporon oleaginosus was analyzed, reaching 172.95 ± 61.31 mg/100 g oil in a lab-scale bioreactor. Using the squalene monooxygenase inhibitor terbinafine, cellular squalene was significantly increased to 2169 ± 262 mg/100 g SCO, while the yeast remained highly oleaginous. Further, SCO from a 1000 L scale production was chemically refined. The squalene content in the deodorizer distillate (DD) was found to be higher than that in DD from typical vegetable oils. Overall, this study demonstrates squalene as a value-added compound in SCO from C. oleaginosus for application in food and cosmetics without the use of genetic modifications.
KW - Cutaneotrichosporon oleaginosus
KW - fermentation
KW - industrial biotechnology
KW - process optimization
KW - single-cell oil
KW - squalene
KW - value-added compound
KW - vertical farming
UR - http://www.scopus.com/inward/record.url?scp=85162197481&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.3c01703
DO - 10.1021/acs.jafc.3c01703
M3 - Article
AN - SCOPUS:85162197481
SN - 0021-8561
VL - 71
SP - 8540
EP - 8550
JO - Journal of agricultural and food chemistry
JF - Journal of agricultural and food chemistry
IS - 22
ER -