Use of gene-modified keratinocytes and fibroblasts to enhance regeneration in a full skin defect

Background: With the development of cell-based gene transfer techniques, genetically modified human keratinocytes (Kc) and fibroblasts (Fb) have been proven to be a better choice in wound repair. Methods: This study was designed to construct in one step a gene-modified artificial skin by a genetically engineered Kc expressing PDGF-BB and Fb expressing VEGF₁₆₅ and bFGF. The wound healing effect in a full-thickness wound model was then observed. Unmodified artificial skin served as control. On the post-operative days 7, 14, and 21, residual wound area was calculated and skin wound tissues were subjected to biopsy for further investigation. Results: Compared with unmodified artificial skin, gene-modified artificial skin resulted in a reduced wound contraction and a well-organized human epidermis and better formed dermis. Conclusions: The results suggest that our two-layer, gene-modified artificial skin improved both vascularization and epidermalization for skin regeneration. This technique could bring about a new approach in the treatment of burns and chronic wounds.