Transcriptional changes and the role of ONECUT1 in hPSC pancreatic differentiation

Sandra Heller, Zhijian Li, Qiong Lin, Ryan Geusz, Markus Breunig, Meike Hohwieler, Xi Zhang, Gopika G. Nair, Thomas Seufferlein, Matthias Hebrok, Maike Sander, Cécile Julier, Alexander Kleger, Ivan G. Costa

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

16 Zitate (Scopus)

Abstract

Cell type specification during pancreatic development is tightly controlled by a transcriptional and epigenetic network. The precise role of most transcription factors, however, has been only described in mice. To convey such concepts to human pancreatic development, alternative model systems such as pancreatic in vitro differentiation of human pluripotent stem cells can be employed. Here, we analyzed stage-specific RNA-, ChIP-, and ATAC-sequencing data to dissect transcriptional and regulatory mechanisms during pancreatic development. Transcriptome and open chromatin maps of pancreatic differentiation from human pluripotent stem cells provide a stage-specific pattern of known pancreatic transcription factors and indicate ONECUT1 as a crucial fate regulator in pancreas progenitors. Moreover, our data suggest that ONECUT1 is also involved in preparing pancreatic progenitors for later endocrine specification. The dissection of the transcriptional and regulatory circuitry revealed an important role for ONECUT1 within such network and will serve as resource to study human development and disease.

OriginalspracheEnglisch
Aufsatznummer1298
FachzeitschriftCommunications Biology
Jahrgang4
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - Dez. 2021
Extern publiziertJa

Fingerprint

Untersuchen Sie die Forschungsthemen von „Transcriptional changes and the role of ONECUT1 in hPSC pancreatic differentiation“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren