Time dependence of 13C-13C magnetization transfer in isotropic mixing experiments involving amino acid spin systems

Hugh L. Eaton, Stephen W. Fesik, Steffen J. Glaser, Gary P. Drobny

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

24 Zitate (Scopus)

Abstract

The time dependence of 13C-13C coherence transfer for the aliphatic portions of amino acid side chains has been examined by calculating ideal coherence-transfer functions. These results were compared to experimentally determined cross- and diagonal-peak intensities obtained from a series of 2D 13C TOCSY experiments acquired with 12 mixing times ranging from 0.0 to 34.2 ms on a mixture of uniformly 13C-labeled amino acids. In general, the calculated coherence-transfer functions agreed. Unlike 1H-1H TOCSY experiments, the time dependence of 13C-13C coherence transfer for these spin systems is simple and well characterized due to the large and conformationally independent 13C-13C spin-spin couplings. Spectra obtained at mixing times of 8, 14, 20, and 28 ms cover most features of the coherence-transfer functions such as vanishingly small cross peaks and local maxima and minima. These features, along with the fact that 13C-13C coherence transfer takes place in times which are short compared to relaxation times typical for small proteins, make this experiment ideal for the assignment of protein 13C spectra, which in turn aids in the 1H NMR assignment.

OriginalspracheEnglisch
Seiten (von - bis)452-463
Seitenumfang12
FachzeitschriftJournal of Magnetic Resonance (1969)
Jahrgang90
Ausgabenummer3
DOIs
PublikationsstatusVeröffentlicht - Dez. 1990
Extern publiziertJa

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