TY - JOUR
T1 - The xenoestrogen bisphenol A in the Hershberger assay
T2 - Androgen receptor regulation and morphometrical reactions indicate no major effects
AU - Nishino, Tsuyuki
AU - Wedel, Thilo
AU - Schmitt, Oliver
AU - Schönfelder, Martin
AU - Hirtreiter, Christian
AU - Schulz, Thorsten
AU - Kühnel, Wolfgang
AU - Michna, Horst
PY - 2006/2
Y1 - 2006/2
N2 - We evaluated androgen-like effects of bisphenol A (BPA) using orchiectomized Wistar rats. Animals were treated p.o. either with vehicle or with 3, 50, 200, 500 mg/kg bw/day BPA (n = 13) for 7 days. One group was treated s.c. with 1 mg/kg bw/day testosterone propionate (TP). Flutamide (FL) (3 mg/kg bw/day, p.o.) was used to antagonize androgen effects of the suprapharmacological dose (500 mg/kg bw/day) of BPA. Androgen-like effects of BPA on prostates and seminal vesicles were assessed by the Hershberger assay, densitometric analysis of androgen receptor (AR) immunoreactivity, cell proliferation-index and a morphometric analysis. Absolute weights of prostates and seminal vesicles were not increased by BPA, whereas the relative weights were increased at higher doses of BPA, most likely due to a decrease in body weight. Staining intensity for AR immunoreactivity was increased at low but not at higher doses of BPA in comparison to the orchiectomized rats. BPA at all doses tested did not cause an increase of the cell proliferation-index. Epithelial height and glandular luminal area were increased by low doses of BPA, whereas higher doses caused a decrease of these parameters. The data provide evidence that BPA does not exert major androgenic effects.
AB - We evaluated androgen-like effects of bisphenol A (BPA) using orchiectomized Wistar rats. Animals were treated p.o. either with vehicle or with 3, 50, 200, 500 mg/kg bw/day BPA (n = 13) for 7 days. One group was treated s.c. with 1 mg/kg bw/day testosterone propionate (TP). Flutamide (FL) (3 mg/kg bw/day, p.o.) was used to antagonize androgen effects of the suprapharmacological dose (500 mg/kg bw/day) of BPA. Androgen-like effects of BPA on prostates and seminal vesicles were assessed by the Hershberger assay, densitometric analysis of androgen receptor (AR) immunoreactivity, cell proliferation-index and a morphometric analysis. Absolute weights of prostates and seminal vesicles were not increased by BPA, whereas the relative weights were increased at higher doses of BPA, most likely due to a decrease in body weight. Staining intensity for AR immunoreactivity was increased at low but not at higher doses of BPA in comparison to the orchiectomized rats. BPA at all doses tested did not cause an increase of the cell proliferation-index. Epithelial height and glandular luminal area were increased by low doses of BPA, whereas higher doses caused a decrease of these parameters. The data provide evidence that BPA does not exert major androgenic effects.
KW - Androgen receptor regulation
KW - Bisphenol A
KW - Densitometry
KW - Immunohistochemistry
KW - MIB-5
KW - Morphometry
KW - PCNA
KW - Proliferation markers
KW - Prostate
KW - Seminal vesicle
UR - http://www.scopus.com/inward/record.url?scp=31444444221&partnerID=8YFLogxK
U2 - 10.1016/j.jsbmb.2005.08.019
DO - 10.1016/j.jsbmb.2005.08.019
M3 - Article
C2 - 16406770
AN - SCOPUS:31444444221
SN - 0960-0760
VL - 98
SP - 155
EP - 163
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
IS - 2-3
ER -