TY - JOUR
T1 - The mRNA expression of the members of the IGF-system in bovine corpus luteum during induced luteolysis
AU - Neuvians, T. P.
AU - Pfaffl, M. W.
AU - Berisha, B.
AU - Schams, D.
N1 - Funding Information:
We are deeply grateful for financial support of this project by the “H. Wilhelm Schaumann Stiftung”, Hamburg, Germany, and the German Research Foundation (DFG Scha 257/14). We would like to thank Ch. Fochtmann and Dr. W. Kraetzl for the surgical help in collecting the CL.
PY - 2003/11
Y1 - 2003/11
N2 - The components of the IGF-system were shown to be differentially regulated in bovine antral follicles and corpora lutea (CL) during different stages of the estrous cycle, and to have important functions for specific stages. The aim of this study was to investigate the detailed pattern of mRNA expression of most constituents of the IGF-system and their possible involvement in prostaglandin (PG)F2α-induced luteolysis in the bovine CL. Therefore, cows in the mid-luteal phase (days 8-12) were injected with the PGF2α-analogue Cloprostenol, and CL were collected by transvaginal ovariectomy at 2, 4, 12, 48 and 64 h after PGF2α-injection. Real-time RT-PCR using SYBR Green I detection was employed to determine mRNA expressions of the following factors: ubiquitin (UBQ), insulin-like growth factor I (IGF I), IGF II, IGF-receptor type 1 (IGFR-I), growth hormone receptor (GH-R) and IGF-binding proteins-1-6 (IGFBP-1-6). Total extractable RNA decreased with ongoing luteolysis. IGFBP-1 mRNA was significantly up-regulated at 2 h after PGF2a and maximal at 4 h with a 34-fold increase. IGFBP-5 mRNA was significantly up-regulated after 12h with a maximum of an 11-fold increase at 64h. For GH-R, IGFR-1, IGF II, IGFBP-3 and -4 mRNA expression, we found a significant down-regulation in certain stages. There was a significant up-regulation for IGFBP-2 and -6 mRNA at 64 h after induced luteolysis. There were no significant changes in IGF I mRNA expression. In conclusion, the IGF-system with all its components seems to play an important role in the very complex process of PGF2α-induced luteolysis in bovine CL.
AB - The components of the IGF-system were shown to be differentially regulated in bovine antral follicles and corpora lutea (CL) during different stages of the estrous cycle, and to have important functions for specific stages. The aim of this study was to investigate the detailed pattern of mRNA expression of most constituents of the IGF-system and their possible involvement in prostaglandin (PG)F2α-induced luteolysis in the bovine CL. Therefore, cows in the mid-luteal phase (days 8-12) were injected with the PGF2α-analogue Cloprostenol, and CL were collected by transvaginal ovariectomy at 2, 4, 12, 48 and 64 h after PGF2α-injection. Real-time RT-PCR using SYBR Green I detection was employed to determine mRNA expressions of the following factors: ubiquitin (UBQ), insulin-like growth factor I (IGF I), IGF II, IGF-receptor type 1 (IGFR-I), growth hormone receptor (GH-R) and IGF-binding proteins-1-6 (IGFBP-1-6). Total extractable RNA decreased with ongoing luteolysis. IGFBP-1 mRNA was significantly up-regulated at 2 h after PGF2a and maximal at 4 h with a 34-fold increase. IGFBP-5 mRNA was significantly up-regulated after 12h with a maximum of an 11-fold increase at 64h. For GH-R, IGFR-1, IGF II, IGFBP-3 and -4 mRNA expression, we found a significant down-regulation in certain stages. There was a significant up-regulation for IGFBP-2 and -6 mRNA at 64 h after induced luteolysis. There were no significant changes in IGF I mRNA expression. In conclusion, the IGF-system with all its components seems to play an important role in the very complex process of PGF2α-induced luteolysis in bovine CL.
KW - Bovine
KW - Corpus luteum
KW - IGF
KW - IGFBP
KW - Real-time RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=0347658903&partnerID=8YFLogxK
U2 - 10.1016/j.domaniend.2003.08.002
DO - 10.1016/j.domaniend.2003.08.002
M3 - Article
C2 - 14652136
AN - SCOPUS:0347658903
SN - 0739-7240
VL - 25
SP - 359
EP - 372
JO - Domestic Animal Endocrinology
JF - Domestic Animal Endocrinology
IS - 4
ER -