Structure and ligand recognition of the phosphotyrosine binding domain of Shc

Ming Ming Zhou, Kodimangalam S. Ravichandran, Edward T. Olejniczak, Andrew M. Petros, Robert P. Meadows, Michael Sattler, John E. Harlan, Warren S. Wade, Steven J. Burakoff, Stephen W. Fesik

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

327 Zitate (Scopus)

Abstract

The nuclear magnetic resonance structure of the phosphotyrosine binding (PTB) domain of She complexed to a phosphopeptide reveals an alternative means of recognizing tryosine-phosphorylated proteins. Unlike in SH2 domains, the phosphopeptide forms an antiparallel β-strand with a β-sheet of the protein, interacts with a hydrophobic pocket through the (pY–5) residue, and adopts a β-turn. The PTB domain is structurally similar to pleckstrin homology domains (a β-sandwich capped by an α-helix) and binds to acidic phospholipids, suggesting a possible role in membrane localization.

OriginalspracheEnglisch
Seiten (von - bis)584-592
Seitenumfang9
FachzeitschriftNature
Jahrgang378
Ausgabenummer6557
DOIs
PublikationsstatusVeröffentlicht - 7 Dez. 1995
Extern publiziertJa

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