Abstract
The solvent helps out: Conventional protocols for structural analysis of protein complexes often fail if only sparse experimental data are available. NMR-spectroscopy-derived solvent paramagnetic relaxation enhancements (PREs) from a soluble spin label (picture) significantly improve the structural quality of a 150 kDa protein complex. This general protocol is an efficient approach for structural analysis of large protein complexes in solution.
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 3993-3997 |
| Seitenumfang | 5 |
| Fachzeitschrift | Angewandte Chemie International Edition in English |
| Jahrgang | 50 |
| Ausgabenummer | 17 |
| DOIs | |
| Publikationsstatus | Veröffentlicht - 18 Apr. 2011 |