Spectral hole burning and selection of conformational substates in chromoproteins

J. Friedrich, J. Gafert, J. Zollfrank, J. Vanderkooi, J. Fidy

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

34 Zitate (Scopus)


We investigated spectral holes burnt at 1.5 K into the origins of several tautomeric forms of mesoporphyrin IX-substituted horseradish peroxidase at pH 8 under pressures up to 2 MPa. From the pressure-induced lineshift the compressibility of the apoprotein could be determined. We found that the compressibility changed significantly when measured at different tautomer origins. It was concluded that there must be a correlation between the tautomer configurations of the chromophore and the actual structures of the apoprotein. As a consequence, specific conformational substates of the protein can be selected by optical selection of the associated tautomers.

Seiten (von - bis)1029-1033
FachzeitschriftProceedings of the National Academy of Sciences of the United States of America
PublikationsstatusVeröffentlicht - 1 Feb. 1994
Extern publiziertJa


Untersuchen Sie die Forschungsthemen von „Spectral hole burning and selection of conformational substates in chromoproteins“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren