Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection

Annika Schneider; Sandra Kurz; Katrin Manske; Marianne Janas; Mathias Heikenwälder; Thomas Misgeld; Michaela Aichler; Sebastian Felix Weissmann; Hans Zischka; Percy Knolle; Dirk Wohlleber

doi:10.1038/s41598-019-44922-9

Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection

Annika Schneider, Sandra Kurz, Katrin Manske, Marianne Janas, Mathias Heikenwälder, Thomas Misgeld, Michaela Aichler, Sebastian Felix Weissmann, Hans Zischka, Percy Knolle, Dirk Wohlleber

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

17 Zitate (Scopus)

Abstract

Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.

Originalsprache	Englisch
Aufsatznummer	8492
Fachzeitschrift	Scientific Reports
Jahrgang	9
Ausgabenummer	1
DOIs	https://doi.org/10.1038/s41598-019-44922-9
Publikationsstatus	Veröffentlicht - 1 Dez. 2019

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title = "Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection",

abstract = "Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.",

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doi = "10.1038/s41598-019-44922-9",

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T1 - Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection

AU - Schneider, Annika

AU - Kurz, Sandra

AU - Manske, Katrin

AU - Janas, Marianne

AU - Heikenwälder, Mathias

AU - Misgeld, Thomas

AU - Aichler, Michaela

AU - Weissmann, Sebastian Felix

AU - Zischka, Hans

AU - Knolle, Percy

AU - Wohlleber, Dirk

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.

AB - Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.

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Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection

Abstract

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