TY - JOUR
T1 - Single cell polarity in liquid phase facilitates tumour metastasis
AU - Lorentzen, Anna
AU - Becker, Paul F.
AU - Kosla, Jan
AU - Saini, Massimo
AU - Weidele, Kathrin
AU - Ronchi, Paolo
AU - Klein, Corinna
AU - Wolf, Monika J.
AU - Geist, Felix
AU - Seubert, Bastian
AU - Ringelhan, Marc
AU - Mihic-Probst, Daniela
AU - Esser, Knud
AU - Roblek, Marko
AU - Kuehne, Felix
AU - Bianco, Gaia
AU - O'Connor, Tracy
AU - Müller, Quentin
AU - Schuck, Kathleen
AU - Lange, Sebastian
AU - Hartmann, Daniel
AU - Spaich, Saskia
AU - Groß, Olaf
AU - Utikal, Jochen
AU - Haferkamp, Sebastian
AU - Sprick, Martin R.
AU - Damle-Vartak, Amruta
AU - Hapfelmeier, Alexander
AU - Hüser, Norbert
AU - Protzer, Ulrike
AU - Trumpp, Andreas
AU - Saur, Dieter
AU - Vartak, Nachiket
AU - Klein, Christoph A.
AU - Polzer, Bernhard
AU - Borsig, Lubor
AU - Heikenwalder, Mathias
N1 - Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Dynamic polarisation of tumour cells is essential for metastasis. While the role of polarisation during dedifferentiation and migration is well established, polarisation of metastasising tumour cells during phases of detachment has not been investigated. Here we identify and characterise a type of polarisation maintained by single cells in liquid phase termed single-cell (sc) polarity and investigate its role during metastasis. We demonstrate that sc polarity is an inherent feature of cells from different tumour entities that is observed in circulating tumour cells in patients. Functionally, we propose that the sc pole is directly involved in early attachment, thereby affecting adhesion, transmigration and metastasis. In vivo, the metastatic capacity of cell lines correlates with the extent of sc polarisation. By manipulating sc polarity regulators and by generic depolarisation, we show that sc polarity prior to migration affects transmigration and metastasis in vitro and in vivo.
AB - Dynamic polarisation of tumour cells is essential for metastasis. While the role of polarisation during dedifferentiation and migration is well established, polarisation of metastasising tumour cells during phases of detachment has not been investigated. Here we identify and characterise a type of polarisation maintained by single cells in liquid phase termed single-cell (sc) polarity and investigate its role during metastasis. We demonstrate that sc polarity is an inherent feature of cells from different tumour entities that is observed in circulating tumour cells in patients. Functionally, we propose that the sc pole is directly involved in early attachment, thereby affecting adhesion, transmigration and metastasis. In vivo, the metastatic capacity of cell lines correlates with the extent of sc polarisation. By manipulating sc polarity regulators and by generic depolarisation, we show that sc polarity prior to migration affects transmigration and metastasis in vitro and in vivo.
UR - http://www.scopus.com/inward/record.url?scp=85042774764&partnerID=8YFLogxK
U2 - 10.1038/s41467-018-03139-6
DO - 10.1038/s41467-018-03139-6
M3 - Article
C2 - 29491397
AN - SCOPUS:85042774764
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 887
ER -