TY - JOUR
T1 - Siderophore-mediated zinc acquisition enhances enterobacterial colonization of the inflamed gut
AU - Behnsen, Judith
AU - Zhi, Hui
AU - Aron, Allegra T.
AU - Subramanian, Vivekanandan
AU - Santus, William
AU - Lee, Michael H.
AU - Gerner, Romana R.
AU - Petras, Daniel
AU - Liu, Janet Z.
AU - Green, Keith D.
AU - Price, Sarah L.
AU - Camacho, Jose
AU - Hillman, Hannah
AU - Tjokrosurjo, Joshua
AU - Montaldo, Nicola P.
AU - Hoover, Evelyn M.
AU - Treacy-Abarca, Sean
AU - Gilston, Benjamin A.
AU - Skaar, Eric P.
AU - Chazin, Walter J.
AU - Garneau-Tsodikova, Sylvie
AU - Lawrenz, Matthew B.
AU - Perry, Robert D.
AU - Nuccio, Sean Paul
AU - Dorrestein, Pieter C.
AU - Raffatellu, Manuela
N1 - Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Zinc is an essential cofactor for bacterial metabolism, and many Enterobacteriaceae express the zinc transporters ZnuABC and ZupT to acquire this metal in the host. However, the probiotic bacterium Escherichia coli Nissle 1917 (or “Nissle”) exhibits appreciable growth in zinc-limited media even when these transporters are deleted. Here, we show that Nissle utilizes the siderophore yersiniabactin as a zincophore, enabling Nissle to grow in zinc-limited media, to tolerate calprotectin-mediated zinc sequestration, and to thrive in the inflamed gut. We also show that yersiniabactin’s affinity for iron or zinc changes in a pH-dependent manner, with increased relative zinc binding as the pH increases. Thus, our results indicate that siderophore metal affinity can be influenced by the local environment and reveal a mechanism of zinc acquisition available to commensal and pathogenic Enterobacteriaceae.
AB - Zinc is an essential cofactor for bacterial metabolism, and many Enterobacteriaceae express the zinc transporters ZnuABC and ZupT to acquire this metal in the host. However, the probiotic bacterium Escherichia coli Nissle 1917 (or “Nissle”) exhibits appreciable growth in zinc-limited media even when these transporters are deleted. Here, we show that Nissle utilizes the siderophore yersiniabactin as a zincophore, enabling Nissle to grow in zinc-limited media, to tolerate calprotectin-mediated zinc sequestration, and to thrive in the inflamed gut. We also show that yersiniabactin’s affinity for iron or zinc changes in a pH-dependent manner, with increased relative zinc binding as the pH increases. Thus, our results indicate that siderophore metal affinity can be influenced by the local environment and reveal a mechanism of zinc acquisition available to commensal and pathogenic Enterobacteriaceae.
UR - http://www.scopus.com/inward/record.url?scp=85120612218&partnerID=8YFLogxK
U2 - 10.1038/s41467-021-27297-2
DO - 10.1038/s41467-021-27297-2
M3 - Article
C2 - 34853318
AN - SCOPUS:85120612218
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 7016
ER -