Self-assembly of DNA into nanoscale three-dimensional shapes

Shawn M. Douglas, Hendrik Dietz, Tim Liedl, Björn Högberg, Franziska Graf, William M. Shih

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

2080 Zitate (Scopus)

Abstract

Molecular self-assembly offers a bottom-up route to fabrication with subnanometre precision of complex structures from simple components. DNA has proved to be a versatile building block for programmable construction of such objects, including two-dimensional crystals, nanotubes, and three-dimensional wireframe nanopolyhedra. Templated self-assembly of DNA into custom two-dimensional shapes on the megadalton scale has been demonstrated previously with a multiple-kilobase scaffold strand that is folded into a flat array of antiparallel helices by interactions with hundreds of oligonucleotide staple strands. Here we extend this method to building custom three-dimensional shapes formed as pleated layers of helices constrained to a honeycomb lattice. We demonstrate the design and assembly of nanostructures approximating six shapesmonolith, square nut, railed bridge, genie bottle, stacked cross, slotted crosswith precisely controlled dimensions ranging from 10 to 100 nm. We also show hierarchical assembly of structures such as homomultimeric linear tracks and heterotrimeric wireframe icosahedra. Proper assembly requires week-long folding times and calibrated monovalent and divalent cation concentrations. We anticipate that our strategy for self-assembling custom three-dimensional shapes will provide a general route to the manufacture of sophisticated devices bearing features on the nanometre scale.

OriginalspracheEnglisch
Seiten (von - bis)414-418
Seitenumfang5
FachzeitschriftNature
Jahrgang459
Ausgabenummer7245
DOIs
PublikationsstatusVeröffentlicht - 21 Mai 2009
Extern publiziertJa

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