Selection and characterisation of flavanone 3-hydroxylase mutants of Dahlia, Streptocarpus, Verbena and Zinnia

G. Forkmann, G. Stotz

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

27 Zitate (Scopus)

Abstract

Precursor experiments and chromatographic studies indicate that the hydroxylation of flavanones in the 3-position to dihydroflavonols is blocked in special white-flowering mutants of Dahlia, Streptocarpus, Verbena and Zinnia. The result of our investigations was confirmed in as much as the activity of the enzyme flavanone 3-hydroxylase, which catalyses the conversion of flavanones to dihydroflavonols, could readily be detected in flower extracts of cyanic strains of the four plant species. It was found to be, however, completely absent in flower extracts of the corresponding acyanic mutants. Thus, the interruption of the anthocyanin pathway in these mutants is clearly caused by a lack of this enzyme activity. Similar to the enzymes from other sources, the 3-hydroxylases of Dahlia, Streptocarpus, Verbena and Zinnia are soluble enzymes; they belong to the 2-oxoglutarate-dependent dioxygenases and the reaction is inhibited by ethylenediaminetetraacetic acid, KCN and diethyldithiocarbamate.

OriginalspracheEnglisch
Seiten (von - bis)261-265
Seitenumfang5
FachzeitschriftPlanta
Jahrgang161
Ausgabenummer3
DOIs
PublikationsstatusVeröffentlicht - Mai 1984
Extern publiziertJa

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