Screening by PCR for Defined DNA Sequences in Minimal Amounts of Barley Tissue

K. J. Dehmer, A. Graner, G. Wenzel

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

2 Zitate (Scopus)

Abstract

The potential use of the polymerase chain reaction (PCR) in screening plants for defined DNA sequences was demonstrated in barley (Hordeum vulgare). As templates for PCR, purified DNA as well as DNA from crude cell extracts of different barley tissues can be used. Specific synthesis of the target sequence was possible in both cases. Under optimal conditions, as little as 20 copies of the barley genome, i.e., 20 cells, were sufficient for successful amplification. This amount of DNA is equivalent to significantly less than 1 mg of fresh tissue.

OriginalspracheEnglisch
Seiten (von - bis)70-72
Seitenumfang3
FachzeitschriftPlant Breeding
Jahrgang107
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - Aug. 1991
Extern publiziertJa

Fingerprint

Untersuchen Sie die Forschungsthemen von „Screening by PCR for Defined DNA Sequences in Minimal Amounts of Barley Tissue“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren