TY - JOUR
T1 - Reptin and pontin antagonistically regulate heart growth in zebrafish embryos
AU - Rottbauer, Wolfgang
AU - Saurin, Andrew J.
AU - Lickert, Heiko
AU - Shen, Xuetong
AU - Burns, C. Geoff
AU - Wo, Z. Galen
AU - Kemler, Rolf
AU - Kingston, Robert
AU - Wu, Carl
AU - Fishman, Mark
N1 - Funding Information:
We thank M.H. Boulos, M. McKee, and C. Simpson for excellent technical assistance. We are grateful to H. Clevers for providing TOPFLASH and FOPFLASH plasmids and to F. McCormik for providing the TCF-4E plasmid. This work was supported by grants from NIH – 5R01HL49579, 5R01DK55383, 1RO1HL63206 (M.C.F), DFG Ro 2173/1-1, and BMBF 01GS0108-TP5 (W.R.).
PY - 2002/11/27
Y1 - 2002/11/27
N2 - Organ size is precisely regulated during development, but the control mechanisms remain obscure. We have isolated a mutation in zebrafish, liebeskummer (lik), which causes development of hyperplastic embryonic hearts. lik encodes Reptin, a component of a DNA-stimulated ATPase complex. The mutation activates ATPase activity of Reptin complexes and causes a cell-autonomous proliferation of cardiomyocytes to begin well after progenitors have fashioned the primitive heart tube. With regard to heart growth, β-catenin and Pontin, a DNA-stimulated ATPase that is often part of complexes with Reptin, are in the same genetic pathways. Pontin reduction phenocopies the cardiac hyperplasia of the lik mutation. Thus, the Reptin/Pontin ratio serves to regulate heart growth during development, at least in part via the β-catenin pathway.
AB - Organ size is precisely regulated during development, but the control mechanisms remain obscure. We have isolated a mutation in zebrafish, liebeskummer (lik), which causes development of hyperplastic embryonic hearts. lik encodes Reptin, a component of a DNA-stimulated ATPase complex. The mutation activates ATPase activity of Reptin complexes and causes a cell-autonomous proliferation of cardiomyocytes to begin well after progenitors have fashioned the primitive heart tube. With regard to heart growth, β-catenin and Pontin, a DNA-stimulated ATPase that is often part of complexes with Reptin, are in the same genetic pathways. Pontin reduction phenocopies the cardiac hyperplasia of the lik mutation. Thus, the Reptin/Pontin ratio serves to regulate heart growth during development, at least in part via the β-catenin pathway.
UR - http://www.scopus.com/inward/record.url?scp=18744395748&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(02)01112-1
DO - 10.1016/S0092-8674(02)01112-1
M3 - Article
C2 - 12464178
AN - SCOPUS:18744395748
SN - 0092-8674
VL - 111
SP - 661
EP - 672
JO - Cell
JF - Cell
IS - 5
ER -