TY - JOUR
T1 - Release of the soluble interleukin-6 receptor from human T-cells
AU - Banning, Ursula
AU - Bönig, Halvard
AU - Pafferath, Benedikt
AU - Klein-Vehne, Anne
AU - Burdach, Stefan
AU - Körholz, Dieter
PY - 1998
Y1 - 1998
N2 - The soluble Interleukin-6 receptor (sIL-6R) is capable of confering the Interleukin-6 (IL6) signal onto cells lacking the gp80 ligand binding protein. Here we investigate the release of sIL-6R from T-cells. After 2 h stimulation with PMA, a release of sIL-6R from peripheral human T-cells was observed which was insensitive to the protein synthesis inhibitor cycloheximide. This release was accompanied by a decrease of membrane-bound (rob) IL-tR. After 24 h, however, the observed sIL-6R release did prove to be sensitive to cycloheximide. These results suggest that both shedding and denovo-synthesis may be responsible for the PMA-induced sIL-6R release. In contrast to PMA, neither anti-CD3, a positive, nor IL-10, a negative regulator of IL-6 release from T-cells affected the production of the sIL- 6R. The differential regulation of sIL-6R and IL-6 production by T-cells might be relevant for the immunomodulatory potential of the sIL-6R with respect to the interaction of T- and non-T-cells.
AB - The soluble Interleukin-6 receptor (sIL-6R) is capable of confering the Interleukin-6 (IL6) signal onto cells lacking the gp80 ligand binding protein. Here we investigate the release of sIL-6R from T-cells. After 2 h stimulation with PMA, a release of sIL-6R from peripheral human T-cells was observed which was insensitive to the protein synthesis inhibitor cycloheximide. This release was accompanied by a decrease of membrane-bound (rob) IL-tR. After 24 h, however, the observed sIL-6R release did prove to be sensitive to cycloheximide. These results suggest that both shedding and denovo-synthesis may be responsible for the PMA-induced sIL-6R release. In contrast to PMA, neither anti-CD3, a positive, nor IL-10, a negative regulator of IL-6 release from T-cells affected the production of the sIL- 6R. The differential regulation of sIL-6R and IL-6 production by T-cells might be relevant for the immunomodulatory potential of the sIL-6R with respect to the interaction of T- and non-T-cells.
UR - http://www.scopus.com/inward/record.url?scp=0031899532&partnerID=8YFLogxK
U2 - 10.3109/08820139809070889
DO - 10.3109/08820139809070889
M3 - Article
C2 - 9561917
AN - SCOPUS:0031899532
SN - 0882-0139
VL - 27
SP - 47
EP - 55
JO - Immunological Investigations
JF - Immunological Investigations
IS - 1-2
ER -