Release of the soluble interleukin-6 receptor from human T-cells

Ursula Banning, Halvard Bönig, Benedikt Pafferath, Anne Klein-Vehne, Stefan Burdach, Dieter Körholz

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

4 Zitate (Scopus)

Abstract

The soluble Interleukin-6 receptor (sIL-6R) is capable of confering the Interleukin-6 (IL6) signal onto cells lacking the gp80 ligand binding protein. Here we investigate the release of sIL-6R from T-cells. After 2 h stimulation with PMA, a release of sIL-6R from peripheral human T-cells was observed which was insensitive to the protein synthesis inhibitor cycloheximide. This release was accompanied by a decrease of membrane-bound (rob) IL-tR. After 24 h, however, the observed sIL-6R release did prove to be sensitive to cycloheximide. These results suggest that both shedding and denovo-synthesis may be responsible for the PMA-induced sIL-6R release. In contrast to PMA, neither anti-CD3, a positive, nor IL-10, a negative regulator of IL-6 release from T-cells affected the production of the sIL- 6R. The differential regulation of sIL-6R and IL-6 production by T-cells might be relevant for the immunomodulatory potential of the sIL-6R with respect to the interaction of T- and non-T-cells.

OriginalspracheEnglisch
Seiten (von - bis)47-55
Seitenumfang9
FachzeitschriftImmunological Investigations
Jahrgang27
Ausgabenummer1-2
DOIs
PublikationsstatusVeröffentlicht - 1998
Extern publiziertJa

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