Regions outside the α-crystallin domain of the small heat shock protein Hsp26 are required for its dimerization

Jin Chen, Matthias J. Feige, Titus M. Franzmann, Alexander Bepperling, Johannes Buchner

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

30 Zitate (Scopus)

Abstract

Small heat shock proteins (sHsps) are a ubiquitous family of molecular chaperones. They form homo-oligomers, composed of mostly 24 subunits. The immunoglobulin-like α-crystallin domain, which is flanked by N- and C-terminal extensions, is the most conserved element in sHsps. It is assumed to be the dimeric building block from which the sHsp oligomers are assembled.Hsp26 from Saccharomyces cerevisiae is a well-characterized member of this family. With a view to study the structural stability and oligomerization properties of its α-crystallin domain, we produced a series of α-crystallin domain constructs. We show that a minimal α-crystallin domain can, against common belief, be monomeric and stably folded. Elongating either the N- or the C-terminus of this minimal α-crystallin domain with the authentic extensions leads to the formation of dimeric species. In the case of N-terminal extensions, their population is dependent on the presence of the complete so-called Hsp26 "middle domain". For the C-terminal extensions, the presence of the conserved IXI motif of sHsps is necessary and sufficient to induce dimerization, which can be inhibited by increasing ionic strength. Dimerization does not induce major changes in secondary structure of the Hsp26 α-crystallin domain. A thermodynamic analysis of the monomeric and dimeric constructs revealed that dimers are not significantly stabilized against thermal and chemical denaturation in comparison to monomers, supporting our notion that dimerization is not a prerequisite for the formation of a well-folded Hsp26 α-crystallin domain.

OriginalspracheEnglisch
Seiten (von - bis)122-131
Seitenumfang10
FachzeitschriftJournal of Molecular Biology
Jahrgang398
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - Apr. 2010

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