Quantitative Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded (FFPE) Human Cardiac Tissue

Omid Azimzadeh; Michael J. Atkinson; Soile Tapio

doi:10.1007/978-1-0716-1186-9_33

Quantitative Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded (FFPE) Human Cardiac Tissue

Omid Azimzadeh, Michael J. Atkinson, Soile Tapio

Publikation: Beitrag in Buch/Bericht/Konferenzband › Kapitel › Begutachtung

2 Zitate (Scopus)

Abstract

Clinical tissue archives represent an invaluable source of biological information. Formalin-fixed, paraffin-embedded (FFPE) tissue can be used for retrospective investigation of biomarkers of diseases and prognosis. Recently, the number of studies using proteome profiling of samples from clinical archives has markedly increased. However, the application of conventional quantitative proteomics technologies remains a challenge mainly due to the harsh fixation process resulting in protein cross-linking and protein degradation. In the present chapter, we demonstrate a protocol for label-free proteomic analysis of FFPE tissue prepared from human cardiac autopsies. The data presented here highlight the applicability and suitability of FFPE heart tissue for understanding the molecular mechanism of cardiac injury using a proteomics approach.

Originalsprache	Englisch
Titel	Methods in Molecular Biology
Herausgeber (Verlag)	Humana Press Inc.
Seiten	525-533
Seitenumfang	9
DOIs	https://doi.org/10.1007/978-1-0716-1186-9_33
Publikationsstatus	Veröffentlicht - 2021
Extern publiziert	Ja

Publikationsreihe

Name	Methods in Molecular Biology
Band	2261
ISSN (Print)	1064-3745
ISSN (elektronisch)	1940-6029

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abstract = "Clinical tissue archives represent an invaluable source of biological information. Formalin-fixed, paraffin-embedded (FFPE) tissue can be used for retrospective investigation of biomarkers of diseases and prognosis. Recently, the number of studies using proteome profiling of samples from clinical archives has markedly increased. However, the application of conventional quantitative proteomics technologies remains a challenge mainly due to the harsh fixation process resulting in protein cross-linking and protein degradation. In the present chapter, we demonstrate a protocol for label-free proteomic analysis of FFPE tissue prepared from human cardiac autopsies. The data presented here highlight the applicability and suitability of FFPE heart tissue for understanding the molecular mechanism of cardiac injury using a proteomics approach.",

keywords = "Cross-linking, Formalin-fixed, paraffin-embedded (FFPE), Heart, Label-free, Protein extraction, Proteomics",

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doi = "10.1007/978-1-0716-1186-9_33",

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AU - Atkinson, Michael J.

AU - Tapio, Soile

PY - 2021

Y1 - 2021

N2 - Clinical tissue archives represent an invaluable source of biological information. Formalin-fixed, paraffin-embedded (FFPE) tissue can be used for retrospective investigation of biomarkers of diseases and prognosis. Recently, the number of studies using proteome profiling of samples from clinical archives has markedly increased. However, the application of conventional quantitative proteomics technologies remains a challenge mainly due to the harsh fixation process resulting in protein cross-linking and protein degradation. In the present chapter, we demonstrate a protocol for label-free proteomic analysis of FFPE tissue prepared from human cardiac autopsies. The data presented here highlight the applicability and suitability of FFPE heart tissue for understanding the molecular mechanism of cardiac injury using a proteomics approach.

AB - Clinical tissue archives represent an invaluable source of biological information. Formalin-fixed, paraffin-embedded (FFPE) tissue can be used for retrospective investigation of biomarkers of diseases and prognosis. Recently, the number of studies using proteome profiling of samples from clinical archives has markedly increased. However, the application of conventional quantitative proteomics technologies remains a challenge mainly due to the harsh fixation process resulting in protein cross-linking and protein degradation. In the present chapter, we demonstrate a protocol for label-free proteomic analysis of FFPE tissue prepared from human cardiac autopsies. The data presented here highlight the applicability and suitability of FFPE heart tissue for understanding the molecular mechanism of cardiac injury using a proteomics approach.

KW - Cross-linking

KW - Formalin-fixed, paraffin-embedded (FFPE)

KW - Heart

KW - Label-free

KW - Protein extraction

KW - Proteomics

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DO - 10.1007/978-1-0716-1186-9_33

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C2 - 33421012

AN - SCOPUS:85099721164

T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

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Quantitative Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded (FFPE) Human Cardiac Tissue

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