TY - JOUR
T1 - Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells
AU - Haarer, B. K.
AU - Lillie, S. H.
AU - Adams, A. E.M.
AU - Magdolen, V.
AU - Bandlow, W.
AU - Brown, S. S.
PY - 1990/1
Y1 - 1990/1
N2 - We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Müller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins from other organisms in molecular mass and in the ability to bind to polyproline, retard the rate of actin polymerization, and inhibit hydrolysis of ATP by monomeric actin. Using strains that carry disruptions or deletions of the profilin gene, we have found that, under appropriate conditions, cells can survive without detectable profilin. Such cells grow slowly, are temperature sensitive, lose the normal ellipsoidal shape of yeast cells, often become multinucleate, and generally grow much larger than wild-type cells. In addition, these cells exhibit delocalized deposition of cell wall chitin and have dramatically altered actin distributions.
AB - We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Müller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins from other organisms in molecular mass and in the ability to bind to polyproline, retard the rate of actin polymerization, and inhibit hydrolysis of ATP by monomeric actin. Using strains that carry disruptions or deletions of the profilin gene, we have found that, under appropriate conditions, cells can survive without detectable profilin. Such cells grow slowly, are temperature sensitive, lose the normal ellipsoidal shape of yeast cells, often become multinucleate, and generally grow much larger than wild-type cells. In addition, these cells exhibit delocalized deposition of cell wall chitin and have dramatically altered actin distributions.
UR - http://www.scopus.com/inward/record.url?scp=0025008805&partnerID=8YFLogxK
U2 - 10.1083/jcb.110.1.105
DO - 10.1083/jcb.110.1.105
M3 - Article
C2 - 2404021
AN - SCOPUS:0025008805
SN - 0021-9525
VL - 110
SP - 105
EP - 114
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 1
ER -